紫外诱变筛选耐氧性Chlamydomonas moewusii产氢藻株  

Screening of Oxygen-Tolerance Phenotypes in Hydrogen-Producing Strains of Chlamydomonas moewusii with Ultraviolet Radiation

作　　者：张彦婷[1] 范晓蕾[1] 李潇萍[1] 郭荣波[1] 

机构地区：[1]中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《华南理工大学学报（自然科学版）》2011年第5期149-153,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(40806062;20877046);山东省自然科学基金青年基金资助项目(ZR2009EQ011);山东省科技攻关项目(2010GHY10504);中国科学院知识创新工程重要方向项目(KGCX2-YW-373-2)

摘　　要：为解决绿藻中氢化酶对O2极为敏感的问题,以具高产氢催化活性的绿藻Chlamydomonas moewusii野生株作为出发藻株,通过紫外辐射,结合甲硝唑及外加氧法进行耐氧性产氢突变株的诱变和筛选,得到4株耐氧性及产氢活性均显著提高的突变藻株Mu-1、Mu-2、Mu-3、Mu-4,并进行了相应的氢化酶体内活性、呼吸活性与光系统Ⅱ光化学活性分析.结果表明,4株突变藻株的氢化酶体内活性约为野生株氢化酶体内活性的1.69、2.86、1.06和1.80倍,突变株经1%(体积分数)O2处理后,残留活性分别从野生株的(20.00±2.47)%提高到(34.40±4.88)%、(32.50±2.85)%、(55.60±3.30)%和(43.10±8.73)%,而Mu-1和Mu-2两株藻在生长过程中的的光合作用与呼吸作用均未发现明显变化.In order to reduce the sensitivity of hydrogenases in green algae to O2,four mutants of Chlamydomonas moewusii trains with improved oxygen-tolerance and hydrogen-evolving activities,namely Mu-1,Mu-2,Mu-3 and Mu-4,were generated via ultraviolet mutagenesis and were screened following an exposure to metronidazole and applied oxygen.Then,the screened strains were characterized by the hydrogenases activity in vivo,the respiration activity and the photosystem Ⅱ activity of algal cells.The results show that the hydrogenase activities in vivo of the four mutants are respectively 1.69,2.86,1.06 and 1.80 times that of the wild type,that the residual activities of the mutants after a treatment with 1% O2（volume fraction） increase respectively from the original（20.00±2.47）% to（34.40±4.88）%,（32.50±2.85）%,（55.60±3.30）% and（43.10±8.73）%,and that,there are no obvious differences in photosynthesis and respiration during the growth of Mu-1,Mu-2 and the wild type.

关 键 词：氢化酶 耐氧性 紫外诱变 CHLAMYDOMONAS moewusii 

分 类 号：Q319.33[生物学—遗传学]