CYP725A4分子序列分析与结构建模  

Sequence Analysis and Structure Modeling of CYP 725A4

作　　者：蒙海林[1,2] 汪建峰[2,3] 王勇[2] 张嗣良[3] 王小宁[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]中国科学院上海生命科学研究院合成生物学重点实验室,上海200032 [3]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华南理工大学学报（自然科学版）》2011年第5期154-159,共6页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31070030);上海市"浦江人才计划"项目(09PJ1403600);上海市"科技创新行动计划"重大科技项目(10dz1910100);中国科学院知识创新工程重大项目(KSCX2-EW-J-12)

摘　　要：CYP725A4在大肠杆菌等原核宿主中难以实现高效功能表达,是制约紫杉醇异源合成的瓶颈之一.文中利用生物信息学方法对该酶分子的序列进行疏水性和跨膜区分析,构建了其分子进化树,在此基础上用同源建模方法构建了其天然态以及切除N端跨膜区后的三级结构,并对CYP725A4实现功能表达所必需的辅助还原酶也进行了天然态和切除N端跨膜区后的三级结构建模.所构建的结构模型经PROCHECK验证具有较好的可靠性.The difficulty in functional expression of CYP725A4 in heterogeneous prokaryotic hosts such as E.coli is a key bottleneck of the heterologous biosynthesis of taxol.In this paper,bioinformatic strategies were employed to analyze the hydrophobic profile and the transmembrane helices of CYP 725A4 sequence,and a molecular phylogenetic tree of CYP 725A4 was constructed.Then,based on the results,the tertiary structures of native and N-terminal truncated CYP 725A4 were reconstructed via the homology modeling,and similar operations were performed for native and truncated versions of cytochrome P450 reductase used for the functional expression of CYP 725A4.PROCHECK results show that the constructed structure models are all reliable.

关 键 词：生物信息学 细胞色素P450酶 序列分析 分子建模 功能表达 

分 类 号：Q811.4[生物学—生物工程]