High universality of matK primers for barcoding gymnosperms  

作　　者：Yan LI Lian-Ming GAO Ram C. POUDEL De-Zhu Li Alan FORREST 

机构地区：[1]Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China [2]Institute of Alpine Economic Plants, Yunnan Academy of Agricultural Sciences, Lijiang 674100, China [3]Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China [4]The Graduate University of Chinese Academy of Sciences, Beijing 100049, China [5]Royal Botanic Garden Edinburgh, Edinburgh EH3 5LR, UK

出　　处：《Journal of Systematics and Evolution》2011年第3期169-175,共7页植物分类学报（英文版）

基　　金：supported by the Research Fund for the Large-Scale Scientific Facilities of Chinese Academy of Sciences(Grant No. 2009-LSF-GBOWS-01);the West Light Programme of the Chinese Academy of Sciences (Grant No. 9223111W1);the Talent Project of YunnanProvince,China (Grant No. 2008PY064);grateful to the Gordon and Betty Moore Foundation (#2535) for funding which supported the development of matK primers described in this paper

摘　　要：DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region. A two-marker combination of matK＋rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working Group. However, there are currently no barcoding primers for matK showing high universality in gymnosperms. We used 57 gymnosperm species representing 40 genera, 11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this study. Primer （1F/724R） of rbcL is proposed here as a universal primer for gymnosperms due to high universality. One of the nine candidate matK primers （Gym_F 1A/Gym_R1A） is proposed as the best ＂universal＂ matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences. A specific matK primer for Ephedra was newly designed in this study, which performed well on the sampled species. The primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region. A two-marker combination of matK＋rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working Group. However, there are currently no barcoding primers for matK showing high universality in gymnosperms. We used 57 gymnosperm species representing 40 genera, 11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this study. Primer （1F/724R） of rbcL is proposed here as a universal primer for gymnosperms due to high universality. One of the nine candidate matK primers （Gym_F 1A/Gym_R1A） is proposed as the best ＂universal＂ matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences. A specific matK primer for Ephedra was newly designed in this study, which performed well on the sampled species. The primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.

关 键 词：DNA barcoding MATK GYMNOSPERM primer universality. 

分 类 号：Q949.661[生物学—植物学] Q949.6