Efficiency of DNA barcodes for species delimitation:A case in Pterygiella Oliv.(Orobanchaceae)  

作　　者：Li-Na DONG Alexandra H. WORTLEY Hong WANG De-Zhu LI Lu LU 

机构地区：[1]Key Laboratory of Biodiversity and Biogeography, and Plant Germplasm and Genomics Centre, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650204, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100039, China [3]Royal Botanic Garden Edinburgh, Edinburgh EH3 5LR, UK

出　　处：《Journal of Systematics and Evolution》2011年第3期189-202,共14页植物分类学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.30970201);the Research Fund for the Large-scale Scientific Facilities of CAS(Grant No.2009-LSF-GBOWS-01)

摘　　要：DNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions （rbcL, matK, and ITS） to diagnose Pterygiella species, and ex- amine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.DNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions （rbcL, matK, and ITS） to diagnose Pterygiella species, and ex- amine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.

关 键 词：compensatory base changes （CBCs） DNA barcode ITS-2 non-monophyletic species Pterygiella Oliv. secondary structure. 

分 类 号：Q948.524.5[生物学—植物学] Q523