Identification of species within Tetrastigma (Miq.) Planch.(Vitaceae) based on DNA barcoding techniques  

作　　者：Yuan-Miao FU Wei-Mei JIANG Cheng-Xin FU 

机构地区：[1]The Key Laboratory of Conservation Biology for Endangered W~ldlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China [2]Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Plant Sciences and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, China

出　　处：《Journal of Systematics and Evolution》2011年第3期237-245,共9页植物分类学报（英文版）

基　　金：supported by the Research Fund for the Large-scale Scientific Facilities of the Chinese Academy of Sciences(Grant No.2009-LSF-GBOWS-01);the National Key Project of Scientific and Technical Supporting Programs Funded by the Ministry of Science and Technology of China(Grant No.2008BAC39B05);the Key Program of Traditional Chinese Medicine Modernization of Zhejiang(Grant No.2006C13077)

摘　　要：Many species of Tetrastigma （Miq.） Planch. （Vitaceae） have long been used as medicinal plants in China, and some are endangered due to overexploitation. Although adulterants are often added to traditional Chinese medicines, there is no reliable or practical method for identifying them. In this study, we used four markers （rbcL, matK, trnH-psbA, and internal transcribed spacer [ITS]） as DNA barcodes to test their ability to distinguish species of Tetrastigma. The results indicated that the best barcode was ITS, which showed significant inter-specific genetic variability, and thus its potential as a DNA barcode for identifying Tetrastigma. Multiple loci provided a greater ability to distinguish species than single loci. We recommend using the combined rbcL＋matK＋ITS barcode for the genus. Phylogenetic trees from each barcode were compared. Analyses using the unweighted pair group method with arithmetic mean discriminated an equal or greater percentage of resolvable species than did neighbor joining, maximum likelihood, or maximum parsimony analyses. Additionally, five medicinal species of Tetrastigma, especially T. hemsleyanum, could be identified precisely using DNA barcoding.Many species of Tetrastigma （Miq.） Planch. （Vitaceae） have long been used as medicinal plants in China, and some are endangered due to overexploitation. Although adulterants are often added to traditional Chinese medicines, there is no reliable or practical method for identifying them. In this study, we used four markers （rbcL, matK, trnH-psbA, and internal transcribed spacer [ITS]） as DNA barcodes to test their ability to distinguish species of Tetrastigma. The results indicated that the best barcode was ITS, which showed significant inter-specific genetic variability, and thus its potential as a DNA barcode for identifying Tetrastigma. Multiple loci provided a greater ability to distinguish species than single loci. We recommend using the combined rbcL＋matK＋ITS barcode for the genus. Phylogenetic trees from each barcode were compared. Analyses using the unweighted pair group method with arithmetic mean discriminated an equal or greater percentage of resolvable species than did neighbor joining, maximum likelihood, or maximum parsimony analyses. Additionally, five medicinal species of Tetrastigma, especially T. hemsleyanum, could be identified precisely using DNA barcoding.

关 键 词：DNA barcoding medicinal plants species identification Tetrastigma. 

分 类 号：S667.2[农业科学—果树学] TP391.44[农业科学—园艺学]