腺苷预处理对缺血再灌注心肌血红素加氧酶-1的影响  

Effects of Adenosine Preconditioning on Heme Oxygenase-1 Expression in Myocardial Ischemia/Reperfusion Injury in Rats

作　　者：何祥虎[1] 王焱林[1] 王成夭[1] 饶艳[1] 柯剑娟[1] 

机构地区：[1]武汉大学中南医院麻醉科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2011年第3期281-283,F0002,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30872426)

摘　　要：目的:观察腺苷预处理对心肌缺血再灌注损伤的影响,探讨血红素加氧酶-1(HO-1)在其中的作用。方法:雄性SD大鼠18只,体重(250±16)g,随机分为3组,每组6只:①对照组(C组),②缺血再灌注组(IR组),③腺苷预处理+缺血再灌注组(AP组)。采用结扎/放松左冠状动脉前降支建立心肌缺血再灌注损伤模型。再灌注结束后,采用免疫组化法检测心肌HO-1变化,检测心肌梗死面积、心肌组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,及血清TNF-α水平。结果:与C组比较,IR组心肌组织MDA含量和血清TNF-α水平升高,SOD活性降低,心肌梗死面积增加(P<0.05),HO-1表达差异无统计学意义(P>0.05);与IR组比较,AP组心肌组织MDA含量和血清TNF-α水平降低,SOD活性增强,心肌梗死面积减少,HO-1表达增加(P<0.05)。结论:腺苷预处理对心肌缺血再灌注损伤的保护作用与HO-1的表达增加有关。Objective： To investigate the role of heme oxygenase-1（HO-1） in adenosine preconditioning against myocardial ischemia/reperfusion injury in rats.Methods： Eighteen male SD rats were randomly divided into three groups（n=6）： control group（group C）,ischemia/reperfusion group（group IR）,and adenosine preconditioning＋ischemia/reperfusion group（group AP）.The left anterior descending coronary artery was ligated for 30 min and loosed for 120 min to establish the model of myocardial ischemia/reperfusion injury.At the end of 120 min reperfusion,the expression of HO-1 in myocardium was measured by immunohistochemistry.The infarct area,MDA content and SOD activity in myocardium and serum TNF-α concentration were determined.Results： Compared with those in group C,the infarct area,myocardial MDA content,and serum TNF-α concentration were increased,SOD activity was decreased（P0.05）,but HO-1 expression had no changes（P0.05） in group IR.Compared with those in group IR,the infarct area,myocardial MDA content and serum TNF-α concentration were decreased（P0.05）,along with increased SOD activity and HO-1 expression（P0.05） in group AP.Conclusion： Adenosine preconditioning protects myocardium against ischemia/reperfusion injury,and up-regulation of HO-1 expression may be involved in this protecting process.

关 键 词：预处理 腺苷 心肌再灌注损伤 血红素加氧酶-1 

分 类 号：R654.2[医药卫生—外科学]