甘肃省冬虫夏草遗传多样性的ISSR分析  

Genetic diversity of Ophiocordyceps sinensis in Gansu Province based on inter-simple sequence repeat (ISSR) analyses

作　　者：朱子雄[1] 谢放[1] 张楠[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,兰州730070

出　　处：《菌物学报》2011年第3期501-507,共7页Mycosystema

摘　　要：对采自甘肃省3个冬虫夏草主产区域6个具有代表性地方的18个样本进行ISSR分析。15条ISSR引物共扩增得到条带清晰并呈多态性的95条谱带,每一引物扩增获得的ISSR条带数在3-9条之间,扩增片段集中在300-3,000bp。基于遗传相似性系数(GS)、不加权成对群算术平均法(UPGMA)构建的系统树分析,可以看出:分离自同一株虫草上不同部位的样本无显著遗传差异;同一地点样本间的遗传分化较小;不同地域的样本间存在着较大的遗传分化,遗传多样性较丰富。同时6个地方的冬虫夏草明显地分为3个区域,而在同一区域不同地方的样本又各聚为独立的一类,揭示了ISSR分子标记在分析冬虫夏草分布上表现出明显的地域性。Inter-simple sequence repeats has been used in analyzing 18 Ophiocordyceps sinensis samples from 6 areas in Gansu Province.For 15 ISSR primers,each individual showed about 95 DNA bands which ranging in size from 300 to 3,000bp.Molecular phylogenetic tree constructed by genetic similarity coefficient （GS）,un-weighted pair group method with arithmetic meanscluster analysis （UPGMA） suggested that the samples isolated from different parts of the same caterpillar have no significant genetic differences;the genetic differentiation of the samples from the same location is not evident while that from different areas is comparatively evident.O.sinensis samples from 6 localities are obviously divided into 3 clustering groups and in the same clustering group there are independent local types.ISSR analysis reveals that O.sinensis shows its genetic variation correlated to its living enviroments.

关 键 词：冬虫夏草 ISSR 遗传分化 

分 类 号：S567.35[农业科学—中草药栽培]