立体定向照射兔坐骨神经后S-100蛋白表达及超微结构变化的研究  

Morphology analysis and immunohistochemical study of S-100 protein of rabbit sciatic nerve after stereotactic radiation

作　　者：鞠文翠[1] 林志雄[2] 王牧民[1] 黄静[1] 娄云峰[1] 

机构地区：[1]洛阳市中心医院放疗科,河南洛阳471008 [2]汕头大学医学院附属肿瘤医院放疗科,广东汕头515031

出　　处：《中华肿瘤防治杂志》2011年第6期413-415,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨周围神经放射性损伤产生的过程和机制。方法:利用立体定向放射外科技术照射新西兰大白兔的右下肢坐骨神经,12只兔随机分为2组(n=6),1组单次等中心剂量80 Gy,2组25 Gy。每组再分为2个小组(n=3),分别在照射后5和7个月处死,取受照射和正常的神经段,行电镜观察和S-100免疫组化染色。结果:80 Gy照射后神经纤维坏死严重,S-100蛋白无表达。25 Gy照射后5个月受照神经电镜下表现为脱髓鞘改变;7个月受照神经髓鞘和轴突均出现明显的变性坏死,同时发现神经再生,两组S-100染色均呈强阳性染色。结论:周围神经的放射性损伤起始于射线直接引起髓鞘和轴突的变性坏死,较低剂量照射后,神经发生变性坏死的同时,神经再生已启动;高剂量照射后的神经损伤不可恢复。OBJECTIVE： To investigate the procedure and mechanism of radio-induced peripheral nerve injury in rabbit by stereotactic radiosurgery. METHODS： Twelve rabbits were randomized into two groups （n=6） whose right sciatic nerves were irradiated by stereotactic radiosurgery with single fraction of 25 Gy or 80 Gy, the left sciatic nerve served as a control. Then each group was divided into two groups（n= 3）, and was euthanized 5 months and 7 months after irradiation respectively. Their ultrastructural and immunohistochemical analyses were performed respectively. RESULTS： The nerve irradiated with 80 Gy showed prominent degener- ation and necrosis of axon and myelin, and S-100 protein almost show- ing no positive expression. Myelinoclasis was observed under the elec- tron microscope 5 months after radiation. Degeneration and necrosis of axon and myelin was found 7 months after radiation, and regeneration existed at the same time. But the S-100 protein showed strong positive expresion. CONCLUSIONS： Radiation can cause degeneration and necrosis of axon and myelin directly. The nerve injury caused by a lower dose is repairable, while by a higher dose is not.

关 键 词：周围神经 坐骨神经 放射性损伤 放射外科手术 

分 类 号：R818.7[医药卫生—放射医学]