牛、梅花鹿和山羊X、Y精子头部面积差异分析  

Analysis on the Differences between X-and Y-sperm Head Area in Bull,Stag and Goat

作　　者：宫昌海 王惠娥[2,3] 高庆华[2,3] 唐继伟[3] 

机构地区：[1]新疆巴州畜牧工作站,库尔勒841000 [2]塔里木大学动物科学学院,新疆阿拉尔843300 [3]新疆生产建设兵团塔里木畜牧科技重点实验室,阿拉尔843300

出　　处：《中国草食动物》2011年第3期9-11,共3页China Herbivores

基　　金：国家自然科学基金项目(30760170);国家科技支撑计划课题子课题(2008BADB2B05-1-3)

摘　　要：用流式细胞仪分选有正常繁殖力的公牛(n=3)、公鹿(n=3)、公山羊(n=1)的精液,获得高纯度(≥91%)X和Y细管冷冻精液,对解冻后的X和Y精子进行常规染色制片,采用Motic Images Advanced 3.2软件自动测量X精子和Y精子头部面积。结果显示,公牛X精子的头部面积35.84μm2±4.12μm2,极显著高于Y精子(34.81μm2±3.72μm2)(P<0.01);梅花鹿X精子的头部面积33.29μm2±2.93μm2,极显著高于Y精子(32.90μm2±3.25μm2)(P<0.01);山羊X精子的头部面积28.53μm2±3.16μm2,也极显著高于Y精子(28.07μm2±3.19μm2)(P<0.01);不同分选纯度的公牛精液X精子之间、Y精子之间头部面积差异均不显著(P>0.05)。结论:牛、梅花鹿、山羊X精子头部面积均极显著高于Y精子。Flow sorted frozen-thaw sperm from bulls（n=3,purity≥ 99 %）,goat（n=1,purity≥ 94 %）,sika stags（n=3,purity≥ 91 %） with proven fertility were conventionally stained,and their X-and Y-sperm head areas were measured by Motic Images Advanced 3.2 software.The results showed that the X-sperm head area of bull（35.84 μm2± 4.12 μm2） was significantly higher than the Y-sperm head area（34.81 μm2± 3.72 μm2）（P 0.01）,the difference of head areas between X-and Y-sperm was 2.96 %;The X-sperm head area of sika stags（33.29 μm2±2.93 μm2） was significantly higher than the Y-sperm head area（32.90 μm2±3.25 μm2）（P 0.01）,the difference was 1.18 %;The X sperm head area of goat（8.53 μm2±3.16 μm2） was significantly higher than the Y-sperm head area（28.07 μm2±3.19 μm2）（P 0.01）,the difference was 1.35 %.It concluded that the head area of X-sperm in bull,sika stag and goat was significantly higher than that of the Y-sperm.

关 键 词：牛 公梅花鹿 公山羊 精子 流式细胞仪 头部面积 

分 类 号：S814.2[农业科学—畜牧学]