四川藏鸡保种群遗传多样性研究  

Genetic diversity of the conservation population of tibetan chicken in sichuan province

作　　者：白雪[1] 杨孔[1] 吴梦玲[1] 张建漂[1] 谢均 赵晓刚 阿则 仁巴 董平 铁比 代兴强 李云南 

机构地区：[1]西南民族大学生命科学与技术学院高原动物遗传育种与繁殖教育部重点实验室、青藏高原动物遗传资源保护与利用四川省重点实验室,成都610041 [2]四川甘孜州乡城县农牧局,动物疫病预防控制中心,草原家改站,乡城627850

出　　处：《四川大学学报（自然科学版）》2011年第3期703-707,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省青年基金(08ZQ026-027);西南民族大学中央高校基本科研业务费专项(09NZYZJ03),西南民族大学人才引进基金

摘　　要：利用6对藏鸡微卫星引物对四川甘孜州乡城县藏鸡保种群进行了遗传多样性研究.结果表明:6个微卫星座位中共检测到31个等位基因,单个座位的等位基因数为2～10个,平均等位基因数为5.16个.各座位PIC值分布范围在0.177～0.700之间,平均PIC值为0.537,6个微卫星位点在藏鸡中存在多态性.各座位的表观杂合度在0.220～0.980之间,期望杂合度H在0.198～0.745之间,等位基因频率的范围为1%～89%.本文研究结果表明藏鸡保种群的遗传杂合度较高,遗传多样性丰富.To understand the population genetic diversity of tibetan chicken from Alangcneng county in Sichuan province, six pairs of microsatellite primers were used to detect the genetic diversity of the conservation-population of Tibetan chickens from Xiangcheng. The results showed that thirty-one alleles were detected from six microsatellite loci, and the number of alleles of a single locus was between 2 10, with average being 5.16. The PIC value ranged between 0. 177-0. 700, with the average of 0. 537, indicating six microsatellite loci had polymorphism in tibetan chicken. The apparent heterozygosity of every locus was between 0. 220-0. 980, while the expected heterozygosity between 0. 918-0. 745. The frequency value of allele was in the range of 1%-89%.

关 键 词：藏鸡 保种群 微卫星 遗传多样性 

分 类 号：Q145[生物学—生态学]