转IrrE基因甘蓝型油菜对NaCl胁迫的耐受性应答  

The tolerant responses to NaCl Stress in IrrE-transgenic Brassica napus

作　　者：奉斌[1] 代其林[1] 刘婷婷[1] 田霞[1] 龚元亚[1] 孙英坤[1] 杜世章[2] 王劲[1,2] 

机构地区：[1]西南科技大学辐射生物学效应与应用研究中心,绵阳621000 [2]绵阳师范学院基因研究中心,绵阳621000

出　　处：《四川大学学报（自然科学版）》2011年第3期708-712,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅项目(09ZA034)

摘　　要：以转IrrE基因甘蓝型油菜为材料,研究了转IrrE基因甘蓝型油菜幼苗对NaCl胁迫的耐受性应答情况.在200 mmol/L NaCl胁迫下,随着胁迫时间的延长,转IrrE基因和野生型油菜的POD、SOD和CAT三种抗氧化酶活性均增加,其中野生型油菜的三种抗氧化酶在24h后达到峰值,随后逐渐下降,而转IrrE基因油菜的三种抗氧化酶活性却持续增加,并在相同的胁迫时间内均高于野生型油菜的酶活性;同时转IrrE基因油菜的脯氨酸和可溶性蛋白质含量均比野生型油菜的高,但其丙二醛(MDA)的含量比野生型油菜的低.结果表明,IrrE基因作为一种转录因子可能广泛参与了油菜幼苗对NaCl胁迫的耐受性应答过程,从而提高了植物对NaCl胁迫的耐受能力.The tolerant responses to NaCl stress in IrrE-transgenic Brassica napus were studied. The results showed that the activity of peroxidas （POD）, superoxide dismutase （SOD） and catalase （CAT） in transgenic Brassica napus was gradually increased under 200 mmol/L NaCl during 0-48 h, but the activity of POD, SOD and CAT in non-transgenic Brassica napus was increased during 0~24 h and subse- quently decreased after 24 h. The content of proline and the dissoluble proein in transgenic Brassica hapus was higher than non-transgenic Brassica napus, but the content of malonaldehyde（MDA） in transgenic Brassica napus was lower than non-transgenie Brassica napus. Therefore, IrrE gene enhanced the activities of three antioxidant enzymes in transgenic Brassica napus, and then enhanced the tolerance of Brassica napus against NaCl stress.

关 键 词：NACL胁迫 抗氧化酶 IrrE基因 甘蓝型油菜 

分 类 号：Q945[生物学—植物学]