SHIV1157ipd3N4中国恒河猴细胞适应株静脉感染中国恒河猴有效浓度的确定  

Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques

作　　者：丛喆[1] 蒋虹[1] 金光[1] 陈霆[1] 王卫[1] 陶真[1] 姚南[1] 熊竞[1] 吴芳新[1] 陈志伟[2] 魏强[1] 

机构地区：[1]中国医学科学院医学实验动物研究所卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 [2]香港大学李嘉诚医学院艾滋病研究所,中国香港999077

出　　处：《中国比较医学杂志》2011年第4期12-15,共4页Chinese Journal of Comparative Medicine

基　　金：国家十一五科技重大专项课题(2009ZX10004-402,2008ZX10001-015-10)

摘　　要：目的确定SHIV1157ipd3N4静脉途径感染中国恒河猴的有效病毒浓度,明确SHIV1157ipd3N4感染实验猴体内病毒复制和免疫损伤情况。方法 10只正常中国恒河猴分成6组,分别用10倍系列稀释的病毒液1 mL静脉感染,测定血浆病毒载量,CD4+/CD8+,CD4+T淋巴细胞绝对数,分析感染后恒河猴体内病毒复制和免疫损伤情况。结果 5TCID50/mL以上浓度的SHIV1157ipd3N4能通过静脉途径感染中国恒河猴。结论该实验的成功进行为SHIV/中国恒河猴疾病及评价模型的建立奠定了良好的基础,为今后使用此模型评价抗病毒药物或疫苗提供了条件。Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.

关 键 词：SHIV1157ipd3N4 中国恒河猴 模型 动物 AIDS 

分 类 号：R33[医药卫生—人体生理学]