RT-SHIV感染中国恒河猴及体内传代  

RT-SHIV Infected and Passaged in Chinese-Origin Rhesus Monkeys

作　　者：姚南[1] 王卫[1] 丛喆[1] 陈霆[1] 金光[1] 陶真[1] 陈志伟[2] 魏强[1] 

机构地区：[1]中国医学科学院医学实验动物研究所卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 [2]香港大学李嘉诚医学院艾滋病研究所,中国香港999077

出　　处：《中国比较医学杂志》2011年第4期16-20,共5页Chinese Journal of Comparative Medicine

基　　金：“十一五”国家科技重大专项课题(2009ZX10004-307);协和青年基金(RT-SHIV动物模型的建立)

摘　　要：目的了解RT-SHIV感染中国恒河猴的感染特点,研究RT-SHIV在中国恒河猴中传代特点;建立RT-SHIV中国恒河猴动物模型,为评价HIV-1药物有效性提供动物平台。方法选择4只健康恒河猴,其中两只动物经上肢静脉感染RT-SHIV病毒,感染急性期采取外周血分离CD8-PBMC,扩增病毒,将新制备的病毒静脉感染另外两只中国恒河猴,通过监测血浆病毒载量,CD4+/CD8+比值,CD4+T淋巴细胞和B淋巴细胞的绝对数,了解实验猴的感染状态,同时分析病毒RT基因变异情况。结果 4只动物均获得系统性感染,且传代动物急性期表现更为强烈,RT基因在感染和传代的过程中共观察到3个氨基酸的改变。结论本研究为RT-SHIV中国恒河猴模型的建立提供了基础信息。Objective To study the characteristics of RT-SHIV passaging in Chinese rhesus macaques and to establish a RT-SHIV /rhesus animal model providing an animal platform in evaluation of the effectiveness of HIV-1 drugs.Methods Four healthy rhesus macaques selected were negative to SIV,SRV and STLV-1.Two monkeys of them were infected with RT-SHIV intravenously.Blood samples were collected from the infected monkeys at the acute phase and CD8-PBMC were isolated,and then propagated RT-SHIV.The propagated RT-SHIV with PBMC were injected intravenously into the other two monkeys.Viral load in plasma,CD4 ＋ /CD8 ratio and absolute number of CD4 ＋ T lymphocytes and B lymphocytes were detected,and the RT gene variation was analyzed.Results Systemic infection was established in all of the four rhesus macaques,and the two passaged animals demonstrated more severe changes at the acute phase.There were three amino acid changed during infection and passages.Conclusion The results of our study provide basic information for the establishment of RT-SHIV /Chinese-origin rhesus monkeys models.

关 键 词：RT-SHIV 传代 RT基因突变 

分 类 号：R33[医药卫生—人体生理学]