猪链球菌BALB/c小鼠感染模型的初步建立  

Experimental animal models of BALB / c mice infected with Streptococcus suis

作　　者：张奕强[1,2] 刘文倩[1] 刘永生[1] 王印[2] 姚学萍[2] 张杰[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,农业部兽医公共卫生重点开放实验室,兰州730046 [2]四川农业大学动物医学院,动物检疫实验室,雅安625014

出　　处：《中国人兽共患病学报》2011年第5期418-422,共5页Chinese Journal of Zoonoses

基　　金：中波国际科技合作项目(2010DFA32640)资助

摘　　要：目的观察猪链球菌(Streptococcus suis)感染小鼠后的临床、病理变化,为将小鼠作为实验室研究猪链球菌的实验动物提供依据。方法用猪链球菌活菌经腹腔注射感染小白鼠,并做阴性对照,观察其临床症状,剖解病死小鼠,制作病理切片观察病理变化。并对病死小鼠做病原分离,通过培养特性鉴定、生化试验以及PCR鉴定确定其为猪链球菌。结果小鼠确由感染猪链球菌而致死,体内各组织、器官均产生典型的败血症病变。结论小鼠对猪链球菌易感,能够作为实验室研究猪链球菌良好的实验动物。Mice were infected with living Streptococcus suis by celiac injection and the clinical symptoms were observe.The cadavers of the infected mice were anatomized to observe the pathologic changes through pathologic section preparation.According to the results of the cultural characteristics,biochemistry properties and PCR verification,the strain was identified to be Streptococcus suis.Then the mice were died from the infection of Streptococcus suis.All organs and tissues of the mice presented typical septicemic pathologic changes,proving that mouse could be a good material for study of Streptococcus suis.

关 键 词：猪链球菌 BALB/c小鼠模型 病理变化 

分 类 号：S852.611[农业科学—基础兽医学] R535[农业科学—兽医学]