家蝇抗菌物质诱导表达后的电泳分析  

Induction of antibacterial peptide from Musca domestica and preliminary isolation by polyacrylamide gel electrophoresis

作　　者：许兵红[1] 曾莉萍[1] 董卫华[1] 

机构地区：[1]新乡医学院寄生虫学教研室,新乡453003

出　　处：《中国人兽共患病学报》2011年第5期436-438,共3页Chinese Journal of Zoonoses

基　　金：河南省教育厅科研基金资助项目(No.2007310010)

摘　　要：目的探讨家蝇抗菌肽的诱导及电泳分离方法。方法家蝇三龄幼虫针刺体壁诱导后,第48h提取其血淋巴,置于100℃、80℃水浴10s、30s、1min、3min、5min等不同时间后,观察不同温度处理不同时间后的去杂蛋白效果和对溶壁微球菌的抑菌效果,并对各样品进行电泳分离。结果血淋巴100℃水浴10s、30s、1min仍有抑菌活性,处理3min和5min无抑菌活性;80℃水浴30s-5min均有抑菌活性,电泳显示100℃处理3min和5min的样品与其他相比缺少2条蛋白区带。结论针刺诱导的家蝇抗菌肽分离时血淋巴预处理采用80℃水浴1min去杂蛋白效果较好,诱导的血淋巴100℃处理3min和5min即丧失抗菌活性,电泳显示缺少2条区带,此2条差异区带可能为抗菌肽组份。The objective of this study was to induce and preliminarily isolate antibacterial peptide from Musca domestica by mean of polyacrylamide gel electrophoresis.After being induced by pricking for 48 hours,the hemolymph was collected and tested against Micrococcus lysodeikticus,and then the bacteriostatic effects were observed and the diameters of clear inhibitory ring were recorded respectively.After being bathed at 100℃ and 80℃ for 10sec,30sec,1min,3min and 5min,each hemolymph was centrifuged at 10000r/min for 10min to get rid of unwanted proteins,and the supernatant was tested against M.lysodeikticus and displayed by polyacrylamide gel electrophoresis.The hemolymph didn＇t possess antibacterial activity against M.lysodeikticus after being bathed at 100℃ for 3min and 5min,but possessed antibacterial activity in others.It was also found that the two samples which were bathed at 100℃ for 3min and 5min were lack of two special bands from the others.It implies that bathing at 80℃ for 1min and then centrifuging could get rid of unwanted proteins in a large extent,which indicated that the two special bands are the components of the antibacterial peptides induced in the blowfly.

关 键 词：家蝇 抗菌肽 聚丙烯酰胺凝胶电泳 

分 类 号：R384.2[医药卫生—医学寄生虫学]