Rapid growth and sterility of growth hormone gene transgenic triploid carp  

作　　者：YU Fan XIAO Jun LIANG XiangYang LIU ShaoJun ZHOU GongJian LUO KaiKun LIU Yun HU Wei WANG YaPing ZHU ZuoYan 

机构地区：[1]Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China [2]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

出　　处：《Chinese Science Bulletin》2011年第16期1679-1684,共6页

基　　金：supported by the National Key Basic Research Program of China (2007CB109206);the National Special Fund for Research in Public Welfare Sector (200903046-08)

摘　　要：Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.Triploid carp （100%） with 150 （3n=150） chromosomes were obtained by crossing the females of improved tetraploid hybrids （ ♀, 4n=200） of red crucian carp （♀）xcommon carp （♂） with the males of diploid yellow river carp （♂, 2n=100）. The crosses yielded transgenic triploid carp （positive triploid fish, 44.2% of the progeny） and non-transgenic triploid carp （negative triploid fish）. Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season. Morphologically, both the positive and negative triploid fish were similar. They had a spindle-shaped, laterally compressed, steel grey body with two pairs of barbells. Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents. The positive and negative triploid fish were raised in the same pond for 2 years. The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish. The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish. Thus, we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.

关 键 词：三倍体鱼 黄河鲤鱼 非转基因 生长激素基因 不育 组织学检查 横向压缩 量化特征 

分 类 号：S965.116[农业科学—水产养殖] S513[农业科学—水产科学]