Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation  

作　　者：NGEZAHAYO Frederic WANG XiaoLi YU XiaoMing JIANG LiLi CHU YunJie SHEN BaiHua YAN ZhongKai LIU Bao 

机构地区：[1]Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China [2]Department of Agronomy, Jilin Agricultural University, Changchun 130118, China [3]Changchun University of Chinese Traditional Medicine, Changchun 130021, China [4]Baihua Ginseng Cooperation Ltd, Shanghai 200010, China

出　　处：《Chinese Science Bulletin》2011年第16期1685-1690,共6页

基　　金：supported by the National Natural Science Foundation of China (30870178)

摘　　要：Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes,wild and domesticated.Each form differs conspicuously in root phenotype,but can be converted from one type to another by habitat.Here we show that the habi tat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′sites detected by the methylation-sensitive polymorphism(MSAP)marker.The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form.Interestingly,artificially transplanted ginseng plants recreated in both directions the methylation levels(at least in CHG)of their natural counterparts.The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon.Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng.Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes, wild and domesticated. Each form differs conspicuously in root phenotype, but can be converted from one type to another by habitat. Here we show that the habitat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5＇-CCGG-3＇ sites detected by the methylation-sensitive polymorphism （MSAP） marker. The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form. Interestingly, artificially transplanted ginseng plants recreated in both directions the methylation levels （at least in CHG） of their natural counterparts. The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon. Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng.

关 键 词：DNA甲基化 人参植物 相互转化 表型 诱导 东方 甲基化水平 人居 

分 类 号：S567.51[农业科学—中草药栽培]