组织工程复合物腰椎间盘原位植入生物力学稳定性初步研究  

Biomechanical stability of the tissue engineered intervertebral disc implanted in situ in canine

作　　者：徐成[1] 阮狄克[1] 辛洪奎[1] 李超[1] 王超锋[1] 张超[1] 

机构地区：[1]海军总医院骨科,北京100048

出　　处：《生物医学工程与临床》2011年第3期210-213,共4页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金(30730095)

摘　　要：目的 观察组织工程复合物于犬腰椎间盘原位植入后的生物力学稳定性变化.方法 以犬髓核（NP）细胞-聚乳酸-聚羟基乙酸共聚物（PLGA）支架构建组织工程复合物.将24只12个月龄Beagle犬随机分为4组,A组为非手术对照组,B组行腰椎4～5（L4-5）NP摘除,C组行L4-5 NP摘除＋空白支架植入,D组行L4-5NP摘除＋NP细胞-PLGA支架复合物植入.于手术后第4、8、12周处死实验动物取L1～7节段脊柱,使用MTS-858 Mini Ⅱ型生物力学实验机测量标本L4～5节段在纯力偶距下屈伸及旋转动度,行各组间L4～5节段生物力学稳定性比较.结果 B、C、D组L4～5节段生物力学稳定性较A组均有不同程度降低;B组与C组牛物力学特性差异无明显统计学意义（P＞0.05）;而D组与前两组相比稳定性有明显提高（P＜0.05）.结论 NP细胞-PLGA支架复合物腰椎间盘原位植入能够恢复失稳节段的生物力学稳定性.Objective To evaluate the biomechanical stability of the tissue engineered intervertebral disc implanted in sit in canine. Methods The nucleus pulposus cells were cultured and seeded into the three-dimension porous scaffold to construct the engineered implant. Twenty-four Beagles（I-year old） were randomly divided into 4 groups： non-surgical group（A）, L4-5 discectomy group（B）, L4_5 diseectomy with blank scaffold implantation group（C） and L4-5 discectomy with poly（lactic-co-glyeolic acid）（PLGA） scaffold/NP cells composite implantation group （D）. The canines were sacrificed at 4, 8 and 12 weeks after the operation and the spinal L4-7 were collected for further analysis. Ranges of motion in surgical segments were measured using MTS-858 Mini Ⅱ biomechanical machine and the biomechanical stability of L4-5 in each group were evaluated. Results The biomechanical stabilities of group B, C and D decreased to various extents in comparison to that of group A （P 〈 0.05）. There was no difference between group B and C in term of biomechanical stability, whereas biomechanical stability of group D was better than that of group B and C（P 〈 0.05）. Conclusion It is demonstrated that the PLGA scaffold/NP cells composite implantation could enhance the biomechanical stability of the unstable segment.

关 键 词：生物力学稳定性 组织工程 原位植入 聚乳酸-聚羟基乙酸共聚物支架 髓核 犬 

分 类 号：R687.3[医药卫生—骨科学] R318.01[医药卫生—外科学]