低温驯化和外源糖对爪哇伪枝藻冷胁迫的影响  

Influences of low temperature domestication and exterior saccharides on endurance of Scytonema javanicum to cold stress

作　　者：饶本强[1,2] 吴沛沛[2] 李敦海[2] 陈坤[1] 刘永定[2] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]信阳师范学院生命科学学院,信阳464000

出　　处：《生态科学》2011年第2期128-134,共7页Ecological Science

基　　金：淡水生态与生物技术国家重点实验室资助项目(2008FBZ21);中国博士后科学基金资助项目(20100480920);武汉市科技局与内蒙古自治区发改委重大科技产业化专项(200720112031);河南省高校青年骨干教师资助计划项目;信阳师范学院高层次人才科研启动基金项目;信阳师范学院青年骨干教师资助计划项目

摘　　要：低温是影响荒漠藻类生存的重要环境因子。研究了低温驯化和外源糖对爪哇伪枝藻冷胁迫的作用。实验测定了低温和外源糖处理下伪枝藻某些生理活性指标的变化。结果表明,低温胁迫导致伪枝藻生物量和光合活性的显著降低,低温驯化和外源糖在一定程度上对藻细胞叶绿素a含量和光合活性具有促进作用,低温驯化明显提高了细胞内可溶性蛋白的含量,而对丙二醛的含量影响不大。研究还发现,低温驯化明显降低了细胞膜的相对透性,而外源糖对细胞膜的相对透性影响不大。此外,低温驯化促进了伪枝藻胞外多糖的大量合成。研究结果初步证实了低温驯化和外源糖类对于提高伪枝藻的冷胁迫耐受性具有调节作用。Desert algae have a broad distribution in terrestrial environments, and the algae have been proved to be able to well adapt to extreme environments. Low temperature or cold stress is one of important ecological factors influencingsurvival of desert algae. In this study, a typical eyanobacteria species, Scytonemajavanicum （Kiitz.） Born et Flah, was isolated from desert algal crusts and selected as re^eareh material. In the experiments, 2°C, 10°C and 28°C （as control） were selected for temperature treatments, and simultaneously low temperature domestication and exterior sugars such as sucrose and glucose were used to treat the algal cultures under different low temperatures. The results showed that low temperatures resulted in an obvious decrease in biomass and PS II activity in S. javanicum. Low temperature domestication and exterior sugars promoted chlorophyll a content and PS II activity of the alga to some extent, and low temperature domestication obviously increased intracellular production of water-soluble proteins; while no influence on MDA content was observed. In addition, we found that low temperature domestication evidently decreased secretion of EPS, but exterior sugars had no obvious role in relative osmotic activities of S. javanicum. The results suggest that low temperature domestication and exterior sugars may be crucial for tolerance of S. javanicum to low temperature or cold stress.

关 键 词：荒漠藻 爪哇伪枝藻 低温胁迫 低温驯化 外源糖 冷胁迫耐受性 

分 类 号：Q948.1[生物学—植物学]