氧疗对低氧性肺动脉高压大鼠血浆及肺匀浆中U-Ⅱ的影响  

INFLUENCE OF OXYGEN THERAPY ON PLASMA AND LUNG TISSUE HOMOGENATU U-Ⅱ LEVELIN RATS WITH HYPOXIA-INDUCED PULMONARY HYPERTENSION

作　　者：孙艳宏[1] 孙德俊[2] 

机构地区：[1]内蒙古医学院生理教研室,内蒙古呼和浩特010059 [2]内蒙古医学院第三附属医院呼吸与危重症医学科

出　　处：《内蒙古医学院学报》2011年第1期9-12,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自然科学基金项目(200711020911)

摘　　要：目的:研究低氧性肺动脉高压(HPH)大鼠模型血浆和肺匀浆中尾加压素(U-Ⅱ水平的变化,以探讨氧疗在HPH中的作用及意义。方法:雄性Wistar大鼠30只,分为对照组,低氧(14d)组,氧疗(7d)组,每组10只,测定各组体循环平均压(mSAP)、肺动脉平均压(mPAP)、右心室肥大指数(RVHI)即右心室与左心室加室间隔重量之比率[RV/(LV+S)],血浆及肺匀浆U-Ⅱ水平,光镜观察肺动脉结构改变。结果:与对照组相比,低氧组大鼠的mPAP、RV/(LV+S)数值,血浆及肺匀浆U-Ⅱ水平均显著增高(P<0.01);光镜下,肺动脉平滑肌细胞增生,胶原纤维增多,管壁增厚,管腔狭窄。与低氧组相比,氧疗组大鼠的mPAP值,血浆及肺匀浆U-Ⅱ水平均显著降低(P<0.01),RV/(LV+S)无显著差异性(P>0.05);光镜下,管腔狭窄有所缓解。结论:U-Ⅱ参与低氧性肺动脉高压的病理生理过程,氧疗可以降低血浆及肺匀浆U-Ⅱ水平并缓解缺氧引起的肺血管重构。Objective： To investigate the alteration of plasma and lung tissue homogenate Urotensin Ⅱ（U-Ⅱ） levels in rats with hypoxia-induced pulmonary hypertension and to study the role of oxygen therapy in the development of HPH.Methods： Thirty male wistar rats were randomly divided into three groups： control group,group with hypoxia for 14 days and group with oxygen therapy after hypoxia for 7 days,ten rats per group.Mean pulmonary artery pressure（mPAP）,mean Systematic artery pressure（mSAP） and the ratio of the weight of right ventricle to that of left ventricle plus septumwere measured.The plasma and lung tissue homogenate levels of U-Ⅱ were measured.The structure in pulmonary arteries were examined using optical microscope.Results：Compared with the control group,mPAP,RV/（LV＋S）,the plasma and the lung tissues homogenate levels of U-Ⅱ in hypoxia group were significantly higher respectively（P〈0.05）.The smooth muscle cells（SMC）proliferation,the collagen deposition in the vassels wall and every calibre showed marked thickening were observed by optical microscope.Compared with hypoxia group,mPAP,the plasma and the lung tissues homoganate levels of U-Ⅱ oxygen therapy group were significantly lower respectively（P〈0.05） while there were no significant differences about RV/（LV＋S） between the two groups.The thickened caliber was relieved significantly by optical microscope.Conclusion：The chang of U-Ⅱ may be involved in the development of HPH.The plasma and the lung tissues homogenate levels of U-Ⅱ were significantly lower and pulmonary vascular remodeling were relieved by oxygen therapy.

关 键 词：氧疗 尾加压素 低氧 高血压 肺性 

分 类 号：R543.2[医药卫生—心血管疾病]