TNF-α和IL-6在实验性胰性脑病中的作用  

EFFECTS OF TNF-α AND IL-6 ON THE EXPERIMENTAL PANCREATIC ENCEPHALOPATHY

作　　者：吕飞飞[1] 赵海平[1] 欧阳晓晖[1] 许贵存[2] 

机构地区：[1]内蒙古医学院附属医院普外科,内蒙古呼和浩特010050 [2]内蒙古医学院附属医院急诊外科,内蒙古呼和浩特010050

出　　处：《内蒙古医学院学报》2011年第1期13-17,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金项目(20050801909)

摘　　要：目的:探讨肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)在大鼠实验型胰性脑病中对脑组织的作用,为揭示胰性脑病的发病机理和治疗提供实验依据。方法:将36只Wistar大鼠按拆信封法随机分为对照组(n=6)和胰性脑病组(n=10×3)。对照组经颈内动脉注入生理盐水;胰性脑病组经颈内动脉注入磷脂酶A2,复制大鼠胰性脑病模型。对照组大鼠于制模后第1d处死,胰性脑病组分别于造模后第1、3及7 d各处死动物10只,检测脑组织含水量,观察脑微血管内白细胞聚集及附壁现象、脑细胞和神经纤维变化,检测大鼠脑组织匀浆中TNF-α含量、IL-6含量变化。结果:随时间延长,胰性脑病组大鼠脑微血管内白细胞聚集及附壁数目显著增加,神经元细胞水肿及神经纤维脱髓鞘改变明显,脑组织含水量、及TNF-α、IL-6的含量明显增加(P<0.05,P<0.01)。结论:大鼠胰性脑病模型中,TNF-α和IL-6在胰性脑病时脑损害的发生和发展有重要作用。Objective： To study the effect of tumor necrosis factor-α（TNF-α）and Interleukin 6（IL-6） in brian tissue on pancreatic encephalopathy（PE） in rats.Methods：Thirty-six Wistar rats were randomLy divided into control group（n=6） and PE group（n=30）.The control group were injected normal saline by internal carotid artery（0.1mL/100g）and were killed on the first day after the injection.The PE group were injected phospholipases A2（1000u/100g,1000u/0.1mL） by internal carotid artery,to copy animal model of PE in rat and were killed on 1,3,7 day after the injection of PLA2.The changes of brain water contents,leucocytes accumttlafion and adhemion were measured.TNF-α content and IL-6 content were measured in the brain homogenate in rats.Results：In the PE group,brain water contents,leucocytes accumulation and adhension,TNF-α and IL-6 level were increased significantly than that of the control group,（P〈0.05）.Conclusion：In the PE group,TNF-α and IL-6 play important roles on the emergence and development of brain lesion in PE.

关 键 词：胰性脑病 大鼠 TNF-Α IL-6 

分 类 号：R657.5[医药卫生—外科学]