1,6-二磷酸果糖对窒息大鼠肝脏的保护作用  

The protective role of FDP to the liver of asphyxia rats

作　　者：叶若兰[1] 方勇[1] 朱会耕[1] 

机构地区：[1]复旦大学附属华山医院危重病科,上海200040

出　　处：《肝脏》2011年第2期113-115,共3页Chinese Hepatology

摘　　要：目的研究窒息大鼠应用1,6-二磷酸果糖(FDP)后肝组织结构的改变以及超氧化物歧化酶(SOD)、丙二醛(MDA)的表达变化,探讨FDP对窒息大鼠肝脏的保护作用。方法 SD实验大鼠分为4组:A为窒息组,B为FDP-窒息组,C为FDP-窒息-复氧组,D为对照组。大鼠经气管插管后,关闭人工呼吸机5min使呼吸停止,造成窒息模型;呼吸停止5min后重新开启呼吸机120min为窒息-复氧模型;窒息同时尾静脉注射FDP为给药模型。取肝组织,光镜和电镜观察,并测定SOD活性和MDA含量。结果窒息组肝细胞肿胀、变性并有坏死,超微结构见糖原分散,粗面内质网脱颗粒。SOD活性显著降低,MDA含量明显升高。窒息时给予FDP则肝细胞虽仍有肿胀、点状坏死,超微结构见线粒体肿胀,但总体上比窒息组有明显改善;SOD活性虽比对照组降低,但较窒息组已经有了显著升高;MDA也比窒息组显著降低。FDP-缺氧再复氧,虽对SOD/MDA的作用不大,但肝组织学形态明显改善。结论窒息后及时恢复供氧并结合FDP治疗,可减轻大鼠肝组织的病理和病理生理改变,对缺氧肝组织可能有保护作用。Objective To study the protective role of FDP to the liver of asphyxia rats, we detected the levels of SOD and MDA, and the change of tissue structure of the liver of asphyxia rats with or without 1-6 FDP. Methods SD experimental rats were divided into 4 groups： Group A （asphyxia group）, Group B （FDP- asphyxia group）, Group C （FDP-asphyxia– re-oxygenation group） and Group D （Control group）. After endotracheal intubation, respirator was off for 5 minutes in order to induce the rats to be the asphyxia rat model, and then, respirator was on for 120 minutes in order to induce the asphyxia rats to be asphyxia-re-oxygenation rats model. FDP was injected through caudal vein, meanwhile respirator was turned off. At the end of the experiment, we observed the change of liver tissue structure with optical microscope or electron microscope and assayed the level of SOD and MDA. Results In Group A, hepatic cells swelled obviously, degenerated and occurred necrosis, and the ultra-structural organization of the hepatic cells changed： the dispersion of glucogen and the degranulation of rough endoplasmic reticulum. The activity of SOD decreased obviously. The level of MDA increased remarkably. After the injection of FDP, the level of the change of the tissue structure is markedly less than that of the rats without FDP in the Group B. The activity of SOD in Group A was lower than that in Group B. The level of MDA in Group A was lower than that in Group B. After re-oxygenation, the difference between Group C and Group A or B was not significant, but the tissue structure of hepatic cell improved significantly. Conclusion After asphyxia, re-oxygenation with FDP can improve the pathological changes and pathophysiological changes of liver. FDP possessed the protective capability for the liver of asphyxia rats.

关 键 词：肝保护 窒息 超微结构 1 6-二磷酸果糖 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]