Omeprazole decreases magnesium transport across Caco-2 monolayers  

作　　者：Narongrit Thongon Nateetip Krishnamra 

机构地区：[1]Faculty of Allied Health Sciences,Burapha University,Chonburi 20131,Thailand [2]Consortium for Calcium and Bone Research(COCAB) and Department of Physiology,Faculty of Science,Mahidol University,Bangkok 10400,Thailand

出　　处：《World Journal of Gastroenterology》2011年第12期1574-1583,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by The Thailand Research Fund(to Thongon N),No.MRG5380003

摘　　要：AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg 2+ transport and electrical parameters.Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study.An Arrhenius plot was performed to elucidate the activation energy of passive Mg 2+ transport across the Caco-2 monolayers.RESULTS:Both apical to basolateral and basolateral to apical passive Mg 2+ fluxes of omeprazole-treated epithelium were decreased in a dose-and time-dependent manner.Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li +,Na +,K +,Rb +,and Cs + from seriesⅦto seriesⅥof the Eisenman sequence.The Arrhenius plot revealed the higher activation energy for passive Mg 2+ transport in omeprazoletreated epithelium than that of control epithelium,indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg 2+ movement was impeded.CONCLUSION:Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg 2+ transport of Caco-2 monolayers that led to the suppression of passive Mg 2+ absorption.AIM：To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2＋ transport across the intestinal epithelium.METHODS：Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg 2＋ transport and electrical parameters.Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study.An Arrhenius plot was performed to elucidate the activation energy of passive Mg 2＋ transport across the Caco-2 monolayers.RESULTS：Both apical to basolateral and basolateral to apical passive Mg 2＋ fluxes of omeprazole-treated epithelium were decreased in a dose-and time-dependent manner.Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li ＋,Na ＋,K ＋,Rb ＋,and Cs ＋ from seriesⅦto seriesⅥof the Eisenman sequence.The Arrhenius plot revealed the higher activation energy for passive Mg 2＋ transport in omeprazoletreated epithelium than that of control epithelium,indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg 2＋ movement was impeded.CONCLUSION：Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg 2＋ transport of Caco-2 monolayers that led to the suppression of passive Mg 2＋ absorption.

关 键 词：MAGNESIUM PARACELLULAR Proton pump inhibitor Transepithelial Tight junction 

分 类 号：R33[医药卫生—人体生理学]