Protective Effect of Renshen Yangrong Decoction(人参养荣汤) on Bone Marrow against Radiation Injury in Mouse  

作　　者：陈衍智 林飞 庄桂宝 任艳 李萍萍 

机构地区：[1]Key laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Integration of Traditional Chinese and Western Medicine,Peking University School of Oncology,Beijing Cancer Hospital & Institute [2]National Institute for the Control of Pharmaceutical and Biological Products [3]Key laboratory of Carcinogenesis and Translational Research (Ministry of Education)Department of Integration of Traditional Chinese and Western Medicine,Peking University School of Oncology,Beijing Cancer Hospital & Institute

出　　处：《Chinese Journal of Integrative Medicine》2011年第6期453-458,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Scientific and Technologic Items of Traditional Chinese Medicine Administration of Beijing City(No.2004 JingZhongZhongⅣ15)

摘　　要：Objective：To explore the effect of Renshen Yangrong Decoction（人参养荣汤,RYD） in protecting bone marrow from radiation injury.Methods：One hundred and eighty Kuming mice were subjected to the three tests for anti-radiation injury effect evaluation,i.e.the test of peripheral white blood cell（WBC） count, the test of bone marrow nucleated cell count,and the bone marrow micronucleus test,using 60 mice for each test.The mice in each test were divided into 6 groups：the blank control group,the model control group,the positive control group treated by Shiyiwei Shenqi Tablet（十一味参芪片,1.0 g/kg）,and three RYD groups treated with high（42.0 g/kg）,moderate（21.0 g/kg）,and low（10.5 g/kg） doses of crude drugs of RYD,with 10 mice in each group.The treatment was given by gastrogavage perfusion continuously for 7-14 days before mice received ^（60）Co-γray radiation and continued until the end of the experiment.The body weights of the mice were monitored,the changes in peripheral WBC and bone marrow nucleated cells were counted,and the variation in bone marrow micronucleated cells was observed on the respective appointed days.Results：A significant decrease in body weight,peripheral WBC count,and bone marrow nucleated cell count,as well as marked changes in bone marrow micronucleated cells were observed in the mice after radiation,indicating that the radiation injury model was successfully established.As compared with the model control group,the decrease in body weight,peripheral WBC count,and bone marrow nucleated cell count,as well as the increase in bone marrow micronucleus cell count in the high dosage RYD treated group were obviously inhibited or lessened （P0.05 or P0.01）.Conclusion：RYD showed obvious protective effect in mice with bone marrow injury induced by radiation.Objective：To explore the effect of Renshen Yangrong Decoction（人参养荣汤,RYD） in protecting bone marrow from radiation injury.Methods：One hundred and eighty Kuming mice were subjected to the three tests for anti-radiation injury effect evaluation,i.e.the test of peripheral white blood cell（WBC） count, the test of bone marrow nucleated cell count,and the bone marrow micronucleus test,using 60 mice for each test.The mice in each test were divided into 6 groups：the blank control group,the model control group,the positive control group treated by Shiyiwei Shenqi Tablet（十一味参芪片,1.0 g/kg）,and three RYD groups treated with high（42.0 g/kg）,moderate（21.0 g/kg）,and low（10.5 g/kg） doses of crude drugs of RYD,with 10 mice in each group.The treatment was given by gastrogavage perfusion continuously for 7-14 days before mice received ^（60）Co-γray radiation and continued until the end of the experiment.The body weights of the mice were monitored,the changes in peripheral WBC and bone marrow nucleated cells were counted,and the variation in bone marrow micronucleated cells was observed on the respective appointed days.Results：A significant decrease in body weight,peripheral WBC count,and bone marrow nucleated cell count,as well as marked changes in bone marrow micronucleated cells were observed in the mice after radiation,indicating that the radiation injury model was successfully established.As compared with the model control group,the decrease in body weight,peripheral WBC count,and bone marrow nucleated cell count,as well as the increase in bone marrow micronucleus cell count in the high dosage RYD treated group were obviously inhibited or lessened （P0.05 or P0.01）.Conclusion：RYD showed obvious protective effect in mice with bone marrow injury induced by radiation.

关 键 词：Renshen Yangrong Decoction mouse  ANTI-RADIATION peripheral white blood cell count bonemarrow nucleated cell count bone marrow micronucleus count 

分 类 号：R285.5[医药卫生—中药学]