高内涵筛选NF-κB信号通路的技术体系建立  

The Technical System Establishment of NF-κB Signaling Screen by HCS

作　　者：穆蕊[1] 李腾[1] 高彦飞[1] 甄诚[1] 陈亮[1] 于鸣[2] 巩伟丽[1] 李慧艳[1] 

机构地区：[1]国家生物医学分析中心,北京100850 [2]军事医学科学院基础医学研究所细胞免疫学教研室,北京100850

出　　处：《科学技术与工程》2011年第14期3162-3164,共3页Science Technology and Engineering

基　　金：国家自然科学基金(30871234)资助

摘　　要：NF-κB是参与免疫调节、炎症反应、肿瘤发生等过程重要的转录因子,在TNFα、IL-1β、LPS等因子诱导下NF-κB发生核转位并激活其转录活性。尝试利用高内涵筛选(High Content Screening,HCS)和RNA干扰(RNAinterference,RNAi)技术建立NF-κB核转位高通量筛选体系,该体系的应用将有助于发现新的NF-κB信号通路调节因子,为免疫与肿瘤的机制研究提供线索。NF-κB is an important transcription factor that regulate immune reaction,inflammation,tumorigenesis,etc.In response to some cytokines,such as TNFα,IL-1β and LPS,NF-κB translocates from cytoplasm to nuclear and its transcriptional activity is activated.Using high content screening（HCS） and RNA interference（RNAi） technology build a NF-κB high-throughput screening system through quantifing nuclear translocation signals.The application of this screening system will help us to discover the new regulator of NF-κB signaling pathway and reveal the mechanism of immunity and tumorigenesis.

关 键 词：NF-κB高内涵筛选(HCS)RNA干涉(RNAi) 

分 类 号：R392.11[医药卫生—免疫学] Q579.13[医药卫生—基础医学]