碱性成纤维细胞生长因子拮抗庆大霉素肾毒性的形态学研究  

PROTECTIVE EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR (bFGF) ON NEPHROTOXITY CAUSED BY GENTAMICIN

作　　者：黄巨恩[1] 徐志文[1] 陈纡[1] 梁祖鼎[1] 

机构地区：[1]广西医科大学组胚教研室

出　　处：《广西医科大学学报》1999年第3期248-250,共3页Journal of Guangxi Medical University

摘　　要：目的：探讨碱性成纤维细胞生长因子（ｂＦＧＦ）对庆大霉素（ＧＭ）肾毒性的保护作用。方法：豚鼠２０只，随机分为ＧＭ组和ＧＭ＋ｂＦＧＦ组各１０只，ＧＭ组按每只８０ｍｇ／ｋｇｄ－１肌注ＧＭ１７ｄ，停药后继续注射生理盐水６ｄ；ＧＭ＋ｂＦＧＦ组在肌注ＧＭ的同时加肌注２０００Ｕ／ｋｇｄ－１ｂＦＧＦ１７ｄ，停ＧＭ后继续注射ｂＦＧＦ６ｄ。用药前、后称体质量，并做统计学ｔ检验：断头处死动物后取肾皮质做光镜和电镜观察。结果：ＧＭ组动物出现急性肾功能衰竭，用药前、后体质量变化有显著性意义（ｔ＝４．３４６３，Ｐ＜０．０５），形态上肾小管上皮细胞明显损害；ＧＭ＋ｂＦＧＦ组动物无急性肾功能衰竭症状，用药前、后体质量变化无显著性意义（ｔ＝０．３８９３，Ｐ＞０．０５），形态上肾小管上皮细胞基本正常。结论：碱性成纤维细胞生长因子对庆大霉素肾毒性有保护作用。Objective: To explore protective effects of bFGF to nephrotoxity caused by Gentamicin (GM). Methods: 20 guinea pigs were divided randomly into two groups,namely GM group and GM+bFGF group,10 animals in each groupGentamicin was injected to the animals of GM group by muscular route for 17 days at a dosage of 80 mg/kgd1 for each animalSaline solution was injected for 6 days after stopping GM injectionsTo GM+bFGF group,GM and bFGF (2 000 U/kgd1) were injected muscularly to each animal at same time for 17 daysbFGF was used continuously for 6 days after stopping GMAll animals were weighed before and after using drugs for statistic analysisAfter killing animals,renal cortexes were removed for LM and EM observations Results: Acute renal failure appeared in GM group and the change of weights before and after using drug was significant (t=4346 3,P<005) and obvious damages were seen in renal tubules morphologicallyIn contrast to GM group,no acute renal failure appeared in GM+bFGF group and the change of weig hts before and after using drugs was not significant (t=0389 3,P>005) and renal tubules were almost normal morphologically Conclusion: bFGF could provide protective effects on nephrotoxity caused by Gentamicin\=

关 键 词：庆大霉素 肾毒素 豚鼠 BFGF 肾保护 

分 类 号：R692[医药卫生—泌尿科学] R329.24[医药卫生—外科学]