侧脑室注射白细胞介素-2对海马痛单位的影响及其可能机制  

EFFECT OF INTERLEUKIN2 INJECTED INTO THE LATERAL VENTRICLE ON PAIN UNITS OF HIPPOCAMPUS

作　　者：覃筱燕[1] 黄忠致[1] 

机构地区：[1]广西医科大学生理教研室

出　　处：《广西医科大学学报》1999年第3期264-265,共2页Journal of Guangxi Medical University

摘　　要：目的：探讨白细胞介素－２对海马痛单位的影响及其可能机制。方法：本实验采用侧脑室给药法，参照Ｓａｗｙｅｒ兔脑图谱，用ＳＮ－２型推进器以２μｍ／ｓ速度将玻璃微电极插入海马Ｐ２～５，Ｌ３～６，Ｈ６～９处引导神经元自发放电。在Ｐ１Ｌ４，５Ｈ６处埋不锈钢套管，用牙托水配牙托粉固定，作脑室注射用。结果：共引导２０５个海马放电神经元，选择对伤害性刺激起反应的海马痛单位６０个，侧脑室分别注射不同剂量的白细胞介素－２（ＩＬ－２）及其受体阻断剂抗ＩＬ－２单克隆抗体（ＭｃＡｂ）和阿片受体阻断剂纳洛酮后，发现ＩＬ－２能抑制大部分的海马痛单位放电，而此作用被ＭｃＡｂ所阻断。纳洛酮能反转ＩＬ－２对海马痛单位放电的抑制作用（Ｐ＜０．０１）。结论：ＩＬ－２能抑制海马痛神经元的放电，其作用机制可能与阿片受体系统有关。Objective: This study investigates the effect of intracerebroventricular injection of IL2 on the pain unit of HPC and probable mechanism in conscious paralytic rabbits Methods: Refering to Sawyer's The rabbit diencephalon in stereotaxic coordix nates,putting grass microelectrodes into P2￣5,L3￣6,H6￣9 of the HPC in 2 m/s by SN2 to induce discharge of neurons Results: The most discharge electric frequency of HPC decreased significantly after injecting IL2 in differnt dose (50,200,500 pmol/10 l) into the lateral vertricle of rabbits after 30,50,70 min,and this effect was abolished by McAb to IL2Naloxone could abolish the inhibitive effect of descharge electric frequency of IL2 on HPC pain units Conclusion: IL2 could inhibit the discharge electric frequency of HPC pain units;Naloxone could abolish this inhibitive effect of IL2,suggesting that inhibitive effect of IL2 on pain units of HPC is related to opiate receptors\=

关 键 词：白细胞介素2 纳洛酮 海马 痛单位 

分 类 号：R338[医药卫生—人体生理学] R441.1[医药卫生—基础医学]