海南与广西两地微小按蚊杂交试验  

HYBRIDIZATION EXPERIMENTS USING ANOPHELES MINIMUS FROM HAINAN AND GUANGXI

作　　者：石焕焕[1] 田春林[1] 黄若密[1] 何登贤[1] 

机构地区：[1]广西医科大学寄生虫学教研室,南宁530021

出　　处：《广西医科大学学报》1999年第4期399-401,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金! ( 3 946 0 0 74);广西自然科学基金! (桂科配95 180 13 )资助课题

摘　　要：目的 :观察海南微小按蚊与广西微小按蚊之间是否存在生殖隔离。方法 :在海南与广西两地分别采集微小按蚊 ,实验室中建立单雌繁殖线 ,用人工交配方法进行杂交试验 ,观察后代雌蚊的可育性 ;按 Coluzzi方法制备杂种 F1 代雌蚊卵巢营养细胞多线染色体标本 ,观察各区带的联会情况。结果 :各交配组的后代均显示可育 ;杂种 F1 代雌蚊的卵巢营养细胞多线染色体各区正常联会。结论 :海南微小按蚊与广西微小按蚊之间不存在生殖隔离 。Objective:To investigate whether there is any reproductive isolation between the Anopheles minimus from Hainan and Guangxi Methods:The isofemale lines of Anopheles minimus collected from Hainan and Guangxi were set up in the laboratory Hybridization experiments were conducted by forced mating (mosquitoes) in order to observe the reproduction of the desendents The Coluzzi's method was applied to observe the ovarian nurse cell polytene chromosomes from F 1 hybrid females Result:All hybrid desendents were fertile The ovarian nurse cell polytene chromosomes of F 1 hybrid females showed synapsis in all the zones of the chromosomes Conclusion:There isn't any reproductive isolation between the Anopheles minimus from Hainan and Guangxi They are of the same species

关 键 词：微小按蚊 杂交 多线染色体 

分 类 号：R384.1[医药卫生—医学寄生虫学]