Fabrication of porous scaffolds with protein nanogels  

作　　者：ZHU Qi YAN Ming HE Lin ZHU XinYuan LU YunFeng YAN DeYue 

机构地区：[1]State Key Laboratory of Metal Matrix Composites: School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China [2]Department of Chemical & Biomolecular Engineering, University of California, Los Angeles 90055, USA [3]Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Science China Chemistry》2011年第6期961-967,共7页中国科学（化学英文版）

基　　金：support from the National Natural Science Foundation of China (20974062);National Basic Research Program (973 Program, 2009CB930400);Shanghai Leading Academic Discipline Project (B202);China National Funds for Distinguished Young Scientists (21025417)

摘　　要：A novel type of porous scaffold was fabricated from single protein nanogels. The nanogels with single protein as core and crosslinked polymer network as shell were prepared through a two-step procedure including surface acryloylation and in situ radical polymerization. The formation of single protein nanogels was verified by matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer, transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses. Subsequently, the porous scaffolds were fabricated through a solvent evaporating process of aqueous nanogel solutions. The porous scaffolds were characterized by Fourier transform infrared (FTIR), scanning electronic microscopy (SEM), atomic force microscopy (AFM), and fluorescence microscopy. Interestingly, the obtained porous nanogel scaffolds presented multi-level porous morphologies with macro and nano scale pores, providing better spaces and microenvironments than normal macro porous scaffolds. Cell proliferation assay of nanogels showed low cytotoxicity. Considering that both the protein species and polymer constitutes can be pre-designed and adjusted, these multi-level porous nanogel scaffolds are promising candidates for tissue culture applications.A novel type of porous scaffold was fabricated from single protein nanogels. The nanogels with single protein as core and crosslinked polymer network as shell were prepared through a two-step procedure including surface acryloylation and in situ radical polymerization. The formation of single protein nanogels was verified by matrix assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometer, transmission electron microscopy （TEM） and dynamic light scattering （DLS） analyses. Subsequently, the porous scaffolds were fabricated through a solvent evaporating process of aqueous nanogel solutions. The porous scaffolds were characterized by Fourier transform infrared （FTIR）, scanning electronic microscopy （SEM）, atomic force microscopy （AFM）, and fluorescence microscopy. Interestingly, the obtained porous nanogel scaffolds presented multi-level porous morphologies with macro and nano scale pores, providing better spaces and microenvironments than normal macro porous scaffolds. Cell proliferation assay of nanogels showed low cytotoxicity. Considering that both the protein species and polymer constitutes can be pre-designed and adjusted, these multi-level porous nanogel scaffolds are promising candidates for tissue culture applications.

关 键 词：protein nanogel porous scaffold tissue culture 

分 类 号：O633.14[理学—高分子化学] TS202.3[理学—化学]