超干及老化处理对紫花苜蓿种子活力和生理变化的影响  

Effect of Ultra-drying and Accelerated Ageing on Vigor and Physiological Activity of Alfalfa Seeds

作　　者：霍平慧[1] 李剑峰[1] 师尚礼[1] 张淑卿[1] 赵文翰[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《中国草地学报》2011年第3期28-34,共7页Chinese Journal of Grassland

基　　金：国家自然科学基金(31060326);国家科技支撑项目(2007BAD52B06;2008BADB3B07);农业部行业专项(nyhyzx07-022);现代农业产业技术体系建设专项资金

摘　　要：以陇东紫花苜蓿为研究对象,采用室温硅胶干燥法对种子进行不同时间的超干处理,测定室温贮藏与高温老化处理后不同含水量种子的活力及生理变化。结果表明:5.72%含水量的超干处理综合表现最优,该处理下室温贮藏种子的发芽率、发芽势和活力指数分别较对应CK(室温贮藏的未超干种子)高28.91%、30.02%和66.06%,可溶性糖含量、APX活性高22.85%、76.21%,而MDA含量仅为CK的50.15%,TTC相对减少量为CK的95%;老化种子的发芽率、发芽势和活力指数较对应CK(老化处理未超干种子)高26.31%、23.19%和67.09%,可溶性糖含量、APX活性分别高39%、115.27%,MDA含量仅为CK的52.32%,TTC相对减少量为CK的93.05%。说明5.72%种子含水量的超干处理可使苜蓿种子活力保持在较高状态,对苜蓿种子的长期保存和利用有积极作用。Medicago sativa L.cv.Longdong was used as test material,and silica gel was used to study the effect of ultra-drying on vigor and physiological activity of normally stored and aged alfalfa seeds.The results indicated that seeds with moisture content at 5.72% performed best among all the tested indices,the germination rate,germination potential and vigor index were increased by 28.91%,30.02% and 66.06% than the control（un-ultradried seeds that stored at room temperature）,while the above indices of aged seeds were increased by 26.31%,23.19% and 67.09% than the control（un-ultradried but accelerating aged seeds）,respectively;for normally stored seeds,soluble sugar content and APX activity were increased by 22.85% and 76.21% than the control,whereas MDA content was only 50.15% of the control and relative TTC reduction quantity was 95% of the control;for accelerating aged seeds,soluble sugar content and APX activity were increased by 39% and 115.27% than the control,whereas MDA content was only52.32% and relative TTC reduction quantity was 93.05% of the control,indicating that ultra-drying treatment of alfalfa seeds with moisture content at 5.72% helps to maintain seeds vigor,which plays a positive effect on long-term preservation and utilization of alfalfa seeds.

关 键 词：紫花苜蓿 超干处理 老化 种子活力 生理活性 

分 类 号：S541.093[农业科学—作物学] Q945[生物学—植物学]