bFGF/BMP/胶原膜加速颌骨骨折愈合的大体及X线观察  

Prospect of bFGF/BMP/collagen Membrane as a Growth Factor Release Material for Jaw Fracture

作　　者：陈建霖 唐彦峰[2,3] 胡学清 张继忠 张彦 白成志 刘磊[2] 

机构地区：[1]灵武市人民医院口腔科,宁夏灵武750400 [2]四川大学华西口腔医学院口腔颌面外科,四川成都610041 [3]包头中心医院口腔科,内蒙古包头014040

出　　处：《现代生物医学进展》2011年第9期1655-1658,共4页Progress in Modern Biomedicine

基　　金：宁夏银川市科技攻关课题资助项目(2008061);教育部新世纪优秀人才支持计划基金资助项目(NCET-07-0578);四川省杰出青年学科带头人培养基金资助项目(06-ZQ026-008)

摘　　要：目的:了解胶原膜作为生长因子缓释材料治疗颌骨骨折的应用前景。方法:将100μg的rhBMP-2用1ml的bFGF溶液完全溶解;用移液器移出40μl的该溶液,滴加到面积为0.5cm×1cm的胶原膜组织块中,冻干后制成生长因子缓释系统;在12只新西兰大白兔两侧制成人工下颌骨骨折模型,左侧置放bFGF/BMP/胶原膜;右侧均为空白对照;术后2、4、12周行临床大体观察及X线片观察。结果:实验组骨折愈合速度明显快于对照组。术后2周,X线结果显示bFGF/BMP/胶原膜组骨折断端边缘模糊。对照组骨折线明显。术后4周,X线结果显示bFGF/BMP/胶原膜骨折线基本不可见,骨折对位良好,断端边缘基本消失,骨折无错位。对照组骨折下缘可见纤维性骨痂形成,骨折线模糊。术后12周,各组X线结果无差异,骨折部位接近正常骨组织。结论:bFGF/BMP/胶原膜能加速骨折愈合,提高骨折愈合效果。Objective： To explore the possibility of collagen membrane to accelerate mandibular fracture healing. Materials Methods： In this study, 100μg rhBMP-2 solution in 1ml bFGF, removed this study with pipette, dropped in area of 0.5cm×1cm of colla- gen membrane. Then it was dried in the freeze. 12 New Zealand rabbits were randomly divided into two groups, the mandibular fracture model on both sides ware prepared. The left is bFGF / BMP / gelatin sponge group, whereas on the right is the control group. X-ray, HE staining, Masson staining and India ink staining were taken at the 2,4,12 week after surgery. Results： In the experimental group had sig- nificantly faster of fracture healing than the control group. After 2 weeks, X-ray showed that the group of bFGF / BMP / collagen mem- brane had blur edge of fracture fragments, the control group had obvious fracture lines. After 4 weeks, X-ray showed the group of bFGF / BMP / collagen membrane had unvisible fracture line of basic and better fracture healing. The control group showed that the procallus had form and fracture line was fuzzy. After 12weeks, every group had no difference in X-ray result. Conclusion： Based on these findigs, bFGF / BMP / collagen membrane can accelerate fracture healing and improve fracture healing.

关 键 词：下颌骨骨折 碱性成纤维细胞生长因子 骨形成蛋白-2 骨折愈合 胶原膜 

分 类 号：Q95-3[生物学—动物学] Q819