二苯乙烯苷抑制过氧化氢诱导的人脐静脉内皮细胞凋亡  

Effects of TSG on Apoptosis of HUVECs Induced by H_2O_2

作　　者：佟丽[1] 张彩平[1] 龙石银[1] 乔新惠[1] 黄良珠[1] 高细强[1] 田英[1] 

机构地区：[1]南华大学生化与分子生物学教研室,湖南衡阳421001

出　　处：《现代生物医学进展》2011年第9期1675-1679,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30800474);湖南省教育厅项目(08B065;09C832);湖南省科技厅项目(2010SK3037);湘科条字[2009]130-19)资助

摘　　要：目的:研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVECs)凋亡的保护作用。方法:运用四甲基偶氮唑盐还原法(MTT法)和流式细胞术筛选建立细胞凋亡模型的H2O2合适浓度以及检测不同浓度TSG对H2O2诱导HUVECs的增殖率和凋亡率;Hoechst33258染色观察细胞凋亡形态。结果:MTT及流式法筛选300μmol/L为H2O2作用于细胞的最适凋亡浓度。MTT和流式结果显示,与H2O(2300μmol/L)损伤组比较,10μmol/L与100μmol/LTSG预处理组细胞的增殖率增加(P<0.05),凋亡率显著降低(P<0.01);Hoechst33258染色观察TSG能降低H2O2诱导的细胞凋亡,使细胞凋亡数减少。结论:TSG能抑制H2O2诱导的HUVECs凋亡,从而起到保护血管内皮细胞的作用。Objective： To investigate the protective effect of 2,3,5,4＇-tetrahydroxystilbene-2- O-β -D-glucoside （TSG on human umbilical vein endothelial cells （HUVECs） apoptosis induced by H2O2. Methods： HUVECs were treated with H2O2 （100,200,300,400,500μmol/L） for 24 hours, the optimal concentration of H2O2 was selected by MTT and Flow Cytometry. HUVECs weretreatedwithTSG （0.1,1,10,100μmol/L）for24hoursthenexposedtoH2O2 （100,200,300,400,500μmol/L）for24 hours, cellviability rates and apoptosis rates of different concentration of TSG pretreatment group were detected by MTT and Flow Cytometry. Mophology of apoptosis and the protective effect of TSG on HUVECs induced by H2O2 were detected by Hoechst33258 staining. Results： The opti- mal concentration for H2O2 to establish apoptosis model were 300μmol/L. Cell viability rate of TSG pretreatment group （10μmol/L and 100μmol/L） increased （P0.05） while the apoptosis rate de- creased obviously （P0.01） compared with H2O2 group（300μmol/L）. TSG reduced the number of HUVECs apoptosis. Conclusions：TSG may have a protective effect on HUVECs apoptosis induced by H2O2.

关 键 词：二苯乙烯苷 人脐静脉内皮细胞 细胞凋亡 H2O_2 

分 类 号：R541.4[医药卫生—心血管疾病]