哮喘患者诱导痰和外周血CD3^+CD56^+NKT细胞的检测及意义  

Significance of Detecting CD3^+ CD56^+ NKT Cells in Induced Sputum and Peripheral Blood from Patients with Asthma

作　　者：李向阳[1] 何江玲[1] 曾宪升[1] 郑玉兰[1] 吴斌[1] 

机构地区：[1]襄阳市中心医院(襄樊学院附属医院),湖北襄阳441021

出　　处：《现代生物医学进展》2011年第9期1751-1753,1788,共4页Progress in Modern Biomedicine

摘　　要：目的:研究CD3+CD56+NKT细胞在哮喘患者急性发作期诱导痰和外周血中的比例改变,并探讨其临床意义。方法:以28例哮喘急性发作期患者为研究组,22名正常人作为对照组,采用二色直接荧光素标记法和多参数流式细胞仪检测诱导痰和外周血CD3+CD56+NKT细胞的比例,同时检测外周血IL-4、Ig-E及INF-γ等水平。结果:哮喘患者急性发作期诱导痰和外周血CD3+CD56+NKT细胞明显高于健康对照组(P<0.01)。哮喘患者急性发作期外周血IL-4、Ig-E及INF-γ等水平明显高于健康对照组(P<0.05)。哮喘患者外周血中CD3+CD56+NKT细胞比例与IL-4、Ig-E及INF-γ升高成正相关。结论:哮喘患者急性发作期诱导痰和外周血中的CD3+CD56+NKT细胞明显增高,CD3+CD56+NKT细胞可能通过调节IL-4、Ig-E及INF-γ等细胞因子从而在哮喘的发病机制发挥重要作用。Objective： To explore the role of CD3＋ CD56＋ NKT cells in induced sputum and peripheral blood in pathogenesis of acute asthma. Methods： CD3＋ CD56＋ NKT cells in the induced sputum and peripheral blood in 28 patients with acute asthma and in 22 normal subjects were detected by fluorescein labeling method and flow cytometry, at the same time, the levels of IL-4, Ig-E and INF-γ in the peripheral blood were detected. Results： The percentage of CD3＋ CD56＋ NKT cells in 28 patients with acute asthma was much higher than that of induced sputum and peripheral blood in normal subjects （P0.01）. And the levels of IL-4, Ig-E and INF-γ in 28 pa- tients were higher than that of peripheral blood in normal subjects （P0.05）. The percentage of CD3＋ CD56＋ NKT cells were positively correlated with IL-4 and NF-γ . Conclusions： CD3＋ CD56＋NKT cells in the induced sputum and peripheral blood in acute asthma pa- tients was increased, CD3＋ CD56＋ NKT cells perhaps play an important role in the pathogenesis of asthma by modulating IL-4,Ig-E and INF-γ .

关 键 词：哮喘 NKT细胞 免疫调节 流式细胞术 

分 类 号：R562.25[医药卫生—呼吸系统]