盐酸洛拉曲克脂质体的制备及其质量考察  

Preparation and Quality of Nolatrexed Dihydrochloride Liposomes

作　　者：陈斯泽[1] 陈雪梅[2] 汪森明[3] 张积仁[3] 

机构地区：[1]广东药学院附属第一医院肿瘤科,广东广州510080 [2]南方医科大学热带医学与共公卫生学院,广东广州510515 [3]南方医科大学附属珠江医院肿瘤中心,广东广州510282

出　　处：《现代生物医学进展》2011年第9期1779-1781,共3页Progress in Modern Biomedicine

基　　金：全军医药卫生科研指令性课题;No.04T101

摘　　要：目的:制备盐酸洛拉曲克脂质体并考察其理化特性。方法:采用薄膜挤压-硫酸铵梯度法制备盐酸洛拉曲克脂质体,透射电镜及激光粒度分析仪分别观察和检测其粒径大小及分布,通过紫外分光光度法测定包封率及评估体外释药试验。结果:制备的盐酸洛拉曲克脂质体包封率达83.6%±2.37%,粒径103.5±26nm且分布均匀。24h体外释放实验结果提示约有66.5%的盐酸洛拉曲克从脂质体释放出来。结论:新制备的盐酸洛拉曲克脂质体粒径大小均匀,包封率尚有提高空间,具有体外缓慢释药的特性。Objective： To prepare nolatrexed dihydrochloride liposmes （NDL）and detect their correlative parameters. Methods： The nolatrexed dihydrochloride liposomes were prepared by film dispersion-extrusion combined with the ammonium sulphate gradients method. The UV detector with Sephadex G50 was utilized for the determination of the drug entrapping efficiency of liposomal nolatrexed dihydrochloride. The disposition of particle diameter was investigated by transmission electron microscope（TEM） and laser particle ana- lyzer And the entrapment efficiency and characteristic of drug release in vitro were detected by UV method. Results： The mean diameter of the nolatrexed dihydrochloride liposomes was 103.5±26 nm, with the entrapping efficiency of 83.6 %±2.37%. From the drug release experiment, about 66.5% nolatrexed dihydrochloride was released from the lipidosome after 24 hours. Conclusion： The new prepared li- posomes have good characteristics both in distribution and size, and it also can reach the purpose of slow-release effectiveness in-vitro, but the entrapping efficiency percentage still need to be further lifted.

关 键 词：盐酸洛拉曲克 脂质体 粒径 包封率 

分 类 号：R917[医药卫生—药物分析学]