乙型肝炎病毒前 S1/ S2 功能区变化与临床转归的可能联系  

作　　者：张 瑞[1] 顾长海[1] 赵秋敏 王洪莉[1] 王海涛[1] 

机构地区：[1]第三军医大学附属西南医院全军传染病专科中心

出　　处：《第三军医大学学报》1999年第8期550-553,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：研究乙型肝炎病毒（ Ｈ Ｂ Ｖ）前 Ｓ１／ Ｓ２ 基因变异规律及其与 Ｈ Ｂ Ｖ 感染临床转归的关系。方法：选择急性自限性乙型肝炎患者、慢性 Ｈ Ｂ Ｖ 携带者及慢性重型乙型肝炎患者各３ 例，用半巢式 Ｐ Ｃ Ｒ 法从此９ 例 Ｈ Ｂ Ｖ 感染者血清中扩增出前 Ｓ１／ Ｓ２ 片段，并用标准分子生物学方法对 Ｐ Ｃ Ｒ 产物进行克隆和序列分析。每个 Ｈ Ｂ Ｖ 感染者随机选择１０ 个克隆，总共测定了９０ 个克隆。通过核苷酸、氨基酸序列同源性比较分析前 Ｓ１／ Ｓ２ 区内肝细胞结合位点、 Ｂ细胞表位和前 Ｓ１、 Ｓ２ 调控区的缺失状况。结果：不同转归的 Ｈ Ｂ Ｖ 感染者体内的 Ｈ Ｂ Ｖ 前 Ｓ１／ Ｓ２ 变异具有明显不同的特征。急性自限性乙型肝炎：前 Ｓ１／ Ｓ２ 各功能区稳定， Ｔ、 Ｂ细胞表位无变化。慢性 Ｈ Ｂ Ｖ 携带者：前 Ｓ１／ Ｓ２ 各功能区变异较大，存在着 Ｔ、 Ｂ细胞表位的漂移。慢性重型乙型肝炎：优势株内可能存在引起机体免疫性增高的区域。结论：本研究为较全面地了解 Ｈ Ｂ Ｖ 前 Ｓ１／ Ｓ２ 功能区变化与临床转归的可能联系提供了实验依据。Study of the relationship of the changes of H B V pre Sfunction dom ainsw ith clinicaloutcom e of H B V infection Zhang Rui, Gu Changhai, Zhao Qium in, W ang Hongli, W nag Haitao ( Clinical Center of Infectious Disease, Southw est Hospital, Third Military Medical University, Chongqing, 400038)　　 Abstract Objective:　 To study the relationship of the changes of H B V pre Sfunction dom ains w ith theclinicaloutcom e of H B V infection. Methods: 3 groups of H B V infected cases including 3 cases of acute H B V infection w ith com plete recovery, 3 cases of chronic H B V carrier and 3 cases of chronic severe hepatitis B w erestudied. The pre Sgenes w ere am plified from serum sam ples w ith halfnested P C R and the products w ere clonedinto M13 vectors. 10 clones w ere random ly selected from each case and sequenced w ith standard procedures. Atotalof 90 clones w ere sequenced and analyzed w ith D N A hom ology com parison. Results: There w ere differentcharacteristics in H B V infected cases w ith differentoutcom es: ① In case ofacute infection, the great indentity of D N A sequences am ong the clones w ere accom panied w ith stable function dom ains w ithout changes of epitopesfor T and B cells. ② In the chronic H B V carriers, the pre Sfunction dom ains show ed high changes w ith epitopedreifts of T and B cells. ③ In case of chronic severe H B V infection, special changes existed in the function dom ains of B cells, w hich m ay be the cause ofabnorm ally high im m une response and severe liver dam ages. Conclusion: Our findings confirm the factthatthere are relations ofthe changes ofthe pre S1/ S2 function dom ains w iththe clinicaloutcom e of H B V infections. Key w ords　 H B V; Pre S1/ S2; function dom ain; clinicaloutcom e

关 键 词：前S1/S2 功能区 临床转归 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]