栉孔扇贝DAPI带型和PI带型研究  

DAPI-Banding and PI-Banding of Zhikong Scallop (Chlamys farreri)

作　　者：徐俊[1,2] 包振民[1] 任晓亮[1] 王珊[1] 胡丽萍[1] 黄晓婷[1] 

机构地区：[1]中国海洋大学海洋生物遗传育种教育部重点实验室,山东青岛266003 [2]上海市医药学校,上海200135

出　　处：《中国海洋大学学报（自然科学版）》2011年第4期77-80,102,共5页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划项目(2006AA10A408;2010AA10A401);国家自然科学青年基金项目(30901096);中国科学院实验海洋生物学重点实验室开放基金项目(KFN92007NO12)资助

摘　　要：选取栉孔扇贝Chlamys farreri担轮幼虫为材料,采用秋水仙素-低渗-空气干燥法制备染色体标本,应用荧光显带技术,分析了DAPI带和PI带在栉孔扇贝染色体上的分布。DAPI带型结果显示,栉孔扇贝所有染色体上都存在DAPI阳性带,主要分布于传统的着丝粒区和端部区域,另外还存在一些中间区DAPI带及可变带,总带数为62。PI带型结果与DAPI带型结果相似,在所有染色体上都存在PI阳性带。2种带型的阳性带所在位置与异染色质分布区域相吻合。In this paper, the techniques including DAPI-staining and H-staining were used to study the DAPI-banding and PI-banding patterns of Chlarnys farreri. Chromosomes spread from trochophore were prepared by Colchine-hypotonic-air drying methods. The results showed that DAPI-bands displayed in all the chromosomes of C. farreri, and they were mostly distributed in centromeric regions and terminal re- gions. Some interstitial bands and variable bands were also observed. 62 DAPI positive bands were recor- ded in 12 chromosome metaphases. In addition, PI positive bands also displayed in all the chromosomes, and their positions were similar to that of the DAPI-bands. Both the positive DAPI-bands and the positive PI-bands distributed in the heterochromatin regions of C. farreri chromosomes.

关 键 词：栉孔扇贝(Chlamys farreri) 染色体 DAPI带 PI带 

分 类 号：S917.4[农业科学—水产科学]