冠心病患者辅助性T细胞亚群17的变化及意义  

Dynamic changes of Th17 cell in patients with coronary artery disease

作　　者：赵朝[1,2] 苏莹 程曼丽[2] 袁祖贻[1] 刘艳[1] 纪玉强[2] 杨小波[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科,陕西西安710061 [2]西安市第一医院心内科,陕西西安710002 [3]西安市第八医院,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2011年第3期311-314,323,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30871043)~~

摘　　要：目的探讨不同类型冠心病患者外周血辅助性T细胞亚群17(Th17)的变化及临床意义。方法选取20例急性心肌梗死(AMI)患者、23例不稳定性心绞痛(UA)患者、19例稳定性心绞痛(SA)患者,另选22例同期住院的非冠心病患者作为对照组。采用流式细胞术检测外周血Th17细胞的百分率;实时定量PCR检测相关基因IL-17A、RORγt和STAT3 mRNA的表达;ELISA法检测血浆IL-17A的浓度。结果①UA患者和AMI患者外周血中Th17细胞的百分率显著高于SA患者和对照组(P<0.01);②与SA组患者和对照组相比,UA组和AMI组患者Th17细胞相关基因IL-17A、RORγt和STAT3的mRNA表达显著增高或有增高趋势。结论 UA患者和AMI患者外周血中Th17细胞激活,Th17激活可能与斑块的不稳定密切相关。Objective To explore the significance and changes of T helper 17（Th17） cell in different types of coronary artery disease.Methods We recruited 20 patients with acute myocardial infarction（AMI）,23 patients with unstable angina（UA）,19 patients with stable angina（SA） and 22 control subjects without coronary artery disease.The percentage of Th17 cell,plasma IL-17A and mRNA expression of related genes（IL-17A,RORγt and STAT3） in these patients were measured by flow cytometry,ELISA and real-time quantitative PCR,respectively.Results ① The percentage of Th17 cell increased significantly in AMI and UA patients compared with that in SA group and controls（P〈0.01）.② The mRNA expression of Th17 related factors（IL-17A,RORγt and STAT3） was significantly higher or had a tendency to increase in AMI group and UA group than in SA group and controls.Conclusion The activation of Th17 cell exists in AMI patients and UA patients,which may contribute to plaque destabilization

关 键 词：动脉粥样硬化 辅助性T细胞亚群17(Th17) 冠心病 炎症 IL-17A RORΓT STAT3 

分 类 号：R541.4[医药卫生—心血管疾病]