HtrA1和TGF-β1在妊娠期高血压病患者胎盘中的表达  

Expressions of HtrA1 and TGF-β1 in placenta of hypertensive disorder-complicating pregnancy

作　　者：宗璐[1] 苟文丽[1] 郭娜[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2011年第3期355-358,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30872773);陕西省科技攻关项目(No.2009K16-02)~~

摘　　要：目的探讨HtrA1(high temperature requirement A1,HtrA1)和TGF-β1(transforming growth factor-beta 1,TGF-β1)在妊娠期高血压病患者(简称妊高病)胎盘组织中的表达及作用。方法利用免疫组化SABC法检测正常妊娠者60例(对照组)和妊高病者70例(实验组)(其中妊娠期高血压组24例,轻度子痫前期组20例,重度子痫前期组26例)胎盘组织中HtrA1和TGF-β1的蛋白表达,并进行相关性分析。结果 HtrA1在实验组中的表达较对照组显著增高(平均灰度分别为155.05±3.13、152.69±4.11,P<0.01);与对照组比较,轻度子痫前期组、重度子痫前期组均显著增高(分别为154.05±4.96、156.03±5.07,P分别<0.05、<0.01)。TGF-β1在实验组中的表达较对照组显著增高(平均灰度分别为156.33±4.79、152.39±4.84,P<0.01);与对照组比较,轻度子痫前期组、重度子痫前期组均显著增高(分别为159.79±3.92、165.82±3.20,P<0.01)。Pearson相关分析HtrA1与TGF-β1的表达水平在实验组中呈正相关。结论 HtrA1和TGF-β1可能通过某些机制相互调控、协同,两者高表达可能与妊高病的发生发展有关。Objective To study the expressions of high temperature requirement A1（HtrA1） and transforming growth factor-beta 1（TGF-β1） in placenta of patients with hypertensive disorder-complicating pregnancy and their relationship.Methods We recruited 60 normal pregnant women（control group） and 70 women with hypertensive disorder-complicating pregnancy（study group）（including 24 cases of gestational hypertension,20 cases of mild preeclampsia and 26 cases of severe preeclampsia）.The expressions of HtrA1 and TGF-β1 in placenta in each group were detected by immunohistochemical SABC methods.The relationship between HtrA1 and TGF-β1 was determined.Results The level of HtrA1 expression was significantly higher in the study group than in the control group（the mean grey number was 155.05±3.13 and 152.69±4.11,respectively;P〈0.01）.Compared with that in the control group,the mean grey numbers of mild preeclampsia and severe preeclampsia were both significantly higher（154.05±4.96 and 156.03±5.07,respectively;P〈0.05,P〈0.01）.The level of TGF-β1 expression was significantly higher in the study group than in the control group（the mean grey number was 156.33±4.79 and 152.39±4.84,respectively;P〈0.01）.Compared with that in the control group,the mean grey numbers of mild preeclampsia and severe preeclampsia were both obviously higher（159.79±3.92 and 165.82±3.20,respectively;both P〈0.01）.Pearson correlation analysis showed a positive correlation between HtrA1 and TGF-β1 expressions in the study group.Conclusion HtrA1 and TGF-β1 may regulate and synergize with each other.The high expressions of HtrA1 and TGF-β1 may be related to the pathogenesis and development of hypertensive disorder-complicating pregnancy

关 键 词：妊娠期高血压病 HTRA1 TGF-Β1 胎盘 免疫组化 

分 类 号：R714[医药卫生—妇产科学]