五味子对模拟航渡及高强度运动大鼠垂体-睾丸轴及糖代谢的影响  

Effects of fructus schisandrae on the function of the pituitary-testis axis and carbohydrate metabolism in rats undergoing experimental navigation and high-intensity exercise

作　　者：夏萍[1] 孙玲君[1] 王坚[1] 

机构地区：[1]南京大学医学院临床学院/南京军区南京总医院内分泌科,江苏南京210002

出　　处：《中华男科学杂志》2011年第5期472-476,共5页National Journal of Andrology

基　　金：南京军区"十一五"面上课题(06MA132)~~

摘　　要：目的:研究五味子对实验性模拟航渡及高强度运动雄性大鼠睾丸轴功能、超微结构及糖代谢的影响。方法:选取34只SD雄性大鼠,分为安静对照组(A组)、应激对照组(B组)、五味子处理组(C组),B组和C组参照Bedford的训练模式进行训练,分别以生理盐水和五味子灌胃1周,每日2次。实验结束后,取血以放免法测定血清睾酮(T)、皮质酮(CORT)、血清黄体生成素(LH)、葡萄糖(G lu)水平,同时随机取垂体、睾丸组织在电子显微镜下观察其超微结构。结果:①A组血G lu为(5.22±2.13)mmol/L,T为(0.61±0.68)ng/m l,CORT为(4.67±1.19)ng/m l;B组血G lu为(9.41±2.56)mmol/L,较A组水平升高(P<0.01),T为(0.10±0.15)ng/m l,与A组相比水平降低(P<0.01),CORT为(7.25±6.20)ng/m l,与A组无明显差异(P>0.05);C组血CORT为(3.55±3.52)ng/m l,G lu为(5.09±1.64)mmol/L,较B组水平均显著降低(P<0.05,P<0.01),T为(0.11±0.12)ng/m l,较B组无明显变化(P>0.05);LH在3组之间均无显著变化(P>0.05)。②电子显微镜观察发现,B组垂体细胞胞质中分泌颗粒较A组显著减少,C组与B组相比胞质中分泌颗粒的数目明显增多,B组睾丸Leyd ig细胞线粒体肿胀,电子密度增高,嵴减少或消失,C组睾丸Leyd ig细胞中线粒体结构趋向于正常,可见大小不等深染的分泌颗粒。结论:模拟航渡及高强度运动训练抑制睾丸功能并影响细胞内超微结构,灌服五味子对垂体-睾丸轴具有保护作用,同时可降低应激大鼠的血糖水平。Objective： To investigate the influence of fructus schisandrae（FS） on the function of the pituitary-testis axis and carbohydrate metabolism in male rats undergoing experimental navigation and strenuous exercise.Methods： Thirty-four SD rats were randomly allocated into three groups,quiescent control（A）,stress control（B） and FS（C）.Those in Groups B and C received 10 days of Benford＇s high-intensity training,followed by 7 days of intragastric administration of normal saline and FS,respectively.Blood samples were immediately obtained at the end of the experiment for the measurement of the levels of serum testosterone（T）,corticosterone（CORT）,luteinizing hormone（LH） and blood glucose（Glu） by radioimmunoassay.The pituitary gland and testis tissues were also collected for the observation of their ultrastructures under the electron microscope.Results： Group A showed a significantly lower Glu level and a higher T level than B（[5.22±2.13] mmol/L versus [9.41±2.56] mmol/L,and [0.61±0.68] ng/ml versus [0.10±0.15] ng/ml,P0.01）,but there was no significant difference in the CORT level between the two groups（[4.67±1.19] ng/ml versus [7.25±6.20] ng/ml,P0.05）.Compared with Group B,both the Glu and CORT levels were remarkably decreased in Group C（[5.09±1.64] mmol/L and [3.55±3.52] ng/ml,P0.05 and P0.01）,but the T level showed no significant change（[0.11±0.12] ng/ml,P0.05）.And there were no significant differences in the serum LH level among the three groups（P0.05）.Ultrastructural pathology showed a significant reduction of secretory granules in the pituitary cells in Group B as compared with A,and a markedly increased number of granules in the kytoplasm in Group C in comparison with B.Such changes as mitochondrial swelling,increase of electron density and decrease or disappearance of mitochondrial cristae were also found in the Leydig cells of Group B.No significant differences were observed in the testicular cells between Groups and C.Conclusion： Experimental n

关 键 词：五味子 模拟航渡 高强度运动 睾酮 

分 类 号：R285.5[医药卫生—中药学]