酸敏感离子通道在PC12细胞中的表达  

The Expression of Acid Sensing Ion Channels in PC12 Cells Model

作　　者：孙雪[1,2] 王芬[1,2] 胡丽芳[1,2] 李君[1,2] 杨亚萍 刘春风[1,2] 

机构地区：[1]苏州大学附属第二医院神经内科 [2]苏州大学神经科学研究所,215004

出　　处：《中国临床神经科学》2011年第3期225-229,共5页Chinese Journal of Clinical Neurosciences

基　　金：国家自然基金(编号:30870869);苏州市科技发展计划(社会发展及医药)(编号:SS0701)

摘　　要：目的:检测酸敏感离子通道(ASICs)在PC12细胞中的表达情况。方法:采用RT-PCR、Westernblot以及单细胞膜片钳记录方法检测ASICs在培养的PC12细胞中的表达情况。结果:PC12细胞中有ASICs蛋白的表达,且ASICs的6个亚基:ASIC1a,ASIC1b,ASIC2a,ASIC2b,ASIC3,ASIC4均有表达,给予酸诱导能产生电流。结论:PC12细胞上存在ASICs电流,且能被ASICs阻断剂阿米洛利所阻断,这为研究ASICs在神经退行性疾病中的作用提供了较为理想的细胞模型。Aim： To investigate the expression of acid sensing ion channels （ASICs） in PC12 cells. Methods： Expression of ASICs in cultured PC12 cells by RT-PCR, Western blotting and the conventional whole-cell patch recording configuration under voltage-clamp conditions. Results： The expression of ASICs was observed in cultured PC12 cells, including the expression of ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4. Acid activated inward current in PC12 cells. Conclusion： The expression and function of ASICs in PC12 cells were verified by electrophysiological recording. Amiloride, a well-known blocker of ASICs, can block proton-gated currents in PC12 cells reversibly in a dose-dependent manner. PC12 cells may be an ideal cell line for the study of physiological and potential pathological roles of ASICs.

关 键 词：酸敏感离子通道 PC12细胞 阿米洛利 酸中毒 

分 类 号：Q189[生物学—神经生物学]