辽宁省6种常用近交系小鼠10个微卫星位点的遗传质量检测报告  

A Genetic Monitoring Report for Ten Microsatellites DNA Locis of Six Inbred Mice Strains Used in Liaoning Province

作　　者：韩喜彬[1] 苏玉虹[2] 巴彩凤[1] 贺宝军[1] 

机构地区：[1]辽宁医学院实验动物中心,锦州121001 [2]辽宁医学院畜牧兽医学院,锦州121001

出　　处：《中国比较医学杂志》2011年第5期22-25,共4页Chinese Journal of Comparative Medicine

摘　　要：目的利用微卫星技术对辽宁省6种近交系小鼠进行遗传质量分析。方法根据Mouse Genome Database和相关文献选取10个多态信息丰富的位点和引物,进行PCR扩增和PAGE电泳,对小鼠的遗传多态性进行研究。结果不同品系小鼠同一位点的扩增结果表现出多态性,同一品系同一位点表现单态性,所有小鼠的10个位点都处于纯合状态;遗传距离分析表明,C57BL/10与C57BL/6J小鼠之间的遗传距离最近,为0.1021,遗传距离最远的是BALB/c与C57BL/10、C57BL/6J,分别为0.1635和0.1614。结论运用所筛选的10个微卫星位点可以对近交系小鼠进行遗传质量检测,说明该方法具备可行性。Objective To study the genetic quality for six strains of inbred mice in Liaoning Province by microsatellites technology.Methods Ten microsatellites DNA locis and primers were selected in Mouse Genome Database and pertinent literature,and its polymorphism information content was rich,to analyzed on genetic polymorphism of mouse by PCR Amplification and PAGE electrophoresis.Results The same site results of PCR amplification showed polymorphism in different strains,and showed monomorphism in same strains,ten microsatellites DNA locis were homozygous state for all mouse.The genetic distance among C57BL /10 and C57BL /6J was 0.1021,and it was 0.1635 and 0.1614 between BALB /c with C57BL /10 and C57BL /6J.Conclusions It was a feasible way to analyzed genetic monitoring among inbred mouse strains by ten microsatellites DNA locis.

关 键 词：近交系小鼠 遗传质量检测 微卫星 

分 类 号：Q75[生物学—分子生物学] R332[医药卫生—人体生理学]