芒果苷对HepG2.2.15细胞β-arrestins信号通路影响的研究  

Study on the effects of mangiferin on the β-arrestins signal pathway in HepG2.2.15 cells

作　　者：邓家刚[1] 邓静 周文强 兰太进[1] 运晨霞[1] 杨柯[1] 

机构地区：[1]广西中医学院,南宁530001 [2]Harvard Cancer Center,美国Boston

出　　处：《中药药理与临床》2011年第2期22-24,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30271365)

摘　　要：目的:本研究以HepG2.2.15(2215)细胞为细胞模型,探讨芒果苷对2215细胞内β-arrestins信号通路的影响。方法:采用免疫印迹法(Western blotting)分别检测芒果苷对2215细胞中β-arrestins总蛋白含量、β-arrestins磷酸化(p-β-arrestins)水平以及EGF干预后芒果苷对2215细胞中p-β-arrestins水平的影响。结果:在无EGF干预时,芒果苷各剂量组对2215细胞中β-arrestins总蛋白含量及p-β-arrestins水平无影响;在EGF干预后,芒果苷100μg/ml能明显抑制2215细胞中p-β-arrestins水平;芒果苷50μg/ml、25μg/ml对β-arrestins磷酸化程度无影响。结论:抑制2215细胞中p-β-arrestins水平是芒果苷抗HBV和免疫调节作用的可能机制之一。Objective： Based on HepG2.2.15（2215） cells,to investigate the effect of mangiferin on the β-arrestins signal transduction pathway in 2215 cell.Methods ：The protein and phosphoralation levels of β-arrestins in 2215 cell and 2215 treated with EGF were tested with Western blotting.Results： ① Mangiferin had no effect on the protein and phosphoralation levels of β-arrestins in 2215 cell untreated with EGF;②Mangiferin 100μg/ml could obviously inhibit β-arrestins phosphoralation in 2215 cell treated with EGF,but mangiferin 50μg/ml and 25μg/ml had no effect on the phosphoralation level of β-arrestins.Conclusions： Inhibiton to the phosphoralation level of β-arrestins maybe one mechanism of mangiferin to inhibit the duplication of HBV and immunoregulation.

关 键 词：芒果苷 HEPG2.2.15细胞 β-arrestins 信号转导 

分 类 号：R285[医药卫生—中药学]