昆仙胶囊对阿霉素诱导的肾病综合征大鼠的治疗作用  

Therapeutic effects of Kunxian Capsule on adriamycin induced nephritic syndrome in rats

作　　者：严美花[1,2] 张浩军[1] 王康[1] 杨丽平[1] 郑柳涛[1] 罗月会[1] 路晓光[1] 郭景珍[1] 沙洪[1] 付桂香[1] 李克明[1] 李平[2] 

机构地区：[1]中日友好医院临床医学研究所,北京100029 [2]南方医科大学,广州510515

出　　处：《中药药理与临床》2011年第2期100-104,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(No.30973911)

摘　　要：目的:观察昆仙胶囊对阿霉素诱导大鼠肾病综合征的治疗作用。方法:雄性Wistar大鼠尾静脉一次性注射阿霉素6mg/kg建立大鼠肾病综合征模型,分为模型组、激素组(7.5 mg/kg)、昆仙胶囊大(30 g/kg)、中(15 g/kg)及小剂量组(7.5 g/kg),正常对照组大鼠注射等体积生理盐水。连续灌胃52天,每周检测各组大鼠大鼠24 h尿蛋白水平,实验结束时取血测定血清总蛋白、白蛋白、总胆固醇、甘油三酯、尿素氮、肌酐;取肾组织观察病理学改变并对其损害程度进行半定量评分。结果:与模型组比较,昆仙胶囊各剂量组24 h尿蛋白水平显著降低,血清总胆固醇水平显著升高,昆仙胶囊15g/kg、7.5g/kg组能显著降低模型大鼠血清甘油三酯水平,减轻肾小球病理损伤(P<0.01);昆仙胶囊30g/kg和15g/kg也能显著改善模型大鼠肾小管间质损害(P<0.01)。结论:昆仙胶囊对阿霉素诱导的大鼠肾病综合征有良好的治疗作用,其作用机制与改善脂代谢紊乱和减少尿蛋白有关。Objective： To observe the therapeutic effects of Kunxian capsule on rat model of nephrotic syndrome induced by adriamycin.Methods： Rat model of nephritic syndrome was established by caudal vein injection of adriamycin at a dose of 6 mg/kg,Model rats were randomly divided into the following groups： the model group,the glucocorticoid group,the Kunxian capsule group of high dose,the Kunxian capsule group of median dose and the Kunxian capsule group of low dose.while the normal control group rats were injected the same volume of saline.Intragastric administration was performed continually for 52 days.24h urinary protein of all rats was determined every week.All animals were sacrificed at the 53rd day of experiment.Serum total protein,albumin,total cholesterol,triglyceride,blood urea nitrogen and creatinine were measured.Kidney tissues were obtained for pathological examination and semi-quantitative analysis.Results： Compared with the model group,24h urinary protein levels in three Kunxian groups were significantly decreased;the levels of serum total cholesterol in the Kunxian groups were obviously lower than that of the model group（P0.01）;median and low dose Kunxian groups could significantly reduce the levels of serum triglyceride and glomerular injury index of model rats（P0.01）;while high and median dose Kunxian statistically ameliorated tubulointerstitial injury in model rats（P0.01）.Conclusion： Kunxian capsule is effective in the treatment of chronic kidney disease in rat induced by adriamycin,and the mechanisms maybe relate to the improvement of disorder of lipid metabolism and proteinuia.

关 键 词：昆仙胶囊 阿霉素 肾病综合征 蛋白尿 

分 类 号：R285[医药卫生—中药学]