620nm非相干性红光照射对大鼠成骨细胞增殖及分化的影响  

The effects of 620nm noncoherent red light irradiation on proliferation and osteogenic differentiation of rat osteo-blasts

作　　者：彭飞[1] 吴华[1] 郑亚东[1] 徐西强[1] 虞冀哲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《中华物理医学与康复杂志》2011年第5期332-335,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金项目（50477043）

摘　　要：目的研究发光二极管（LED）发射的非相干性红光对大鼠成骨细胞增殖及分化的影响。方法通过体外培养SD大鼠成骨细胞，将620nm波长的LED光源置于细胞上方2cm处，根据红光照射时间将细胞分为对照组、1J／cm。组、2J／cm。组及4J／cm。组，分别给予红光照射0S、150S、300S和600S，红光照射每天1次。采用CCK一8法检测红光照射后第2，4天时各组细胞增殖活性；采用碱性磷酸酶（ALP）活性试剂盒检测照射后第9天时细胞ALP比活性；采用逆转录聚合酶链反应（RT-PCR）检测各组细胞I型胶原（CollcLl）、骨钙素（Bglap）及Runx2的表达情况。结果在红光照射后第4天时，发现1J／cm。组、2J／era。组成骨细胞增殖活性较对照组明显增强（P〈0．05）；在红光照射后第9天时，4J／cm。组ALP比活性较对照组明显提高（P〈0．05）；各红光照射组细胞骨钙素及Runx2表达均较对照组明显增强。结论LED来源的620nm非相干性红光照射能显著促进成骨细胞增殖及分化。Objective To investigate the effects of 620nm noncoherent red light emitted from light-emitting diode （LED） on proliferation and osteogenie differentiation of rat osteoblasts. Methods Osteoblasts were isolated from SD rat and cultured in vitro. The LED was placed at 2cm above the cell layer. Cells were divided into four groups and each group was irradiated for 0s, 150s, 300s and 600s at energy dose of 0, l, 2, and 4J/cm2, respectively. Cells were irradiated once every day. Cellular proliferation activities were evaluated by using cell counting kit-8 （CCK-8） at 2nd and 4th d. The alkaline phosphatase （ALP） ratio activity was evaluated by alkaline phosphatase activity kit at the 9th d, and expressions of osteoblast master genes （ Collod , Bglap and Runx2） were monitored as indicators of osteoblast differentiation by reverse transcription-polymerase chain reaction （RT-PCR） technique. Results In the group irradiated with 1 and 2 J/cm2 pro-liferation activities of osteoblasts enhanced significantly compared with the control group at the 4th d （ P 〈 0.05 ）. In the group irradiated with 4 J/em2 ALP ratio activity elevated significantly compared with the control group at the 9th d （ P 〈 0.05）. In the group of red light irradiation expressions of Bglap and Runx2 enhanced significantly. Conclusion The 620nm nonconhcrent red light emitted from LED can promote proliferation of osteoblasts and enhance osteogenic differentia- tion significantly.

关 键 词：成骨细胞 增殖 分化 光生物调节作用 

分 类 号：R68[医药卫生—骨科学]