子宫颈酸性磷酸酶在恶性子宫颈鳞状上皮细胞中异常表达  

Abnormal expression of cervical acid phosphatase in cervical squamous cell carcinoma

作　　者：胡健力[1,2] 李洪言[3] 魏兆莲[3] 孟刚[4] 方圣云[2,4] 沈玉先[2,4] 

机构地区：[1]安徽医科大学药学院,合肥230032 [2]安徽医科大学生物药物研究所,合肥230032 [3]安徽医科大学第一附属医院,合肥230032 [4]安徽医科大学基础医学院,合肥230032

出　　处：《临床与实验病理学杂志》2011年第5期481-483,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的观察子宫颈酸性磷酸酶(cervical acid phosphatase,CAP)在子宫颈鳞状细胞癌(squamous cell carcinoma,SCC)中的异常表达。方法选择30例确诊为子宫颈SCC的住院患者,手术前采集子宫颈涂片,分别进行传统的巴氏染色和CAP检测。CAP的检测采用FSC-811染色试剂盒,细胞胞质中出现红色物质为CAP阳性。巴氏染色结果的判定采用TBS分级系统。结果正常的子宫颈鳞状上皮细胞中CAP表达为阴性,而正常的子宫颈管细胞中CAP表达阳性;30例诊断为子宫颈SCC的患者子宫颈涂片中CAP均为阳性。典型的CAP细胞内分布呈颗粒状,这可能与溶酶体的分布一致。结论 CAP在子宫颈SCC中的表达或活性增加,提示CAP有可能成为子宫颈SCC的生物标记物。Purpose To investigate the expression of cervical acid phosphatase（CAP） in cervical squamous cell carcinoma（SCC）,and to assess the significance of CAP in the early diagnosis of cervical squamous cell carcinoma.Methods Thirty cervical smears collected from the hospitalized patients were subjected to CAP test and routine Papanicolaou（Pap） staining.CAP was detected by FSC-811 kit,and the appearance of red substance in cytoplasm was regarded as CAP positive.Pap staining was estimated with Bethesda System（2001）（TBS）.Results The CAP in normal cervical squamous cell was negative,but positive in the normal endocervical cells,which can be used to distinguish the normal cervical squamous cells from the normal endocervical cells.However,CAP was positive in all the smears collected from 30 patients with cervical SCC.CAP was also positive in the mononuclear phagocytes.Conclusion CAP abnormally expresses in cervical SCC,which may be used as a potential biomarker of SCC.

关 键 词：子宫颈肿瘤 鳞状细胞 酸性磷酸酶 FSC-811 巴氏染色 

分 类 号：R446.1[医药卫生—诊断学] R737.33[医药卫生—临床医学]