城市大气可吸入颗粒物对大鼠变应性鼻炎模型发病的影响  

Experimental study on the impact of urban inhalable particles on allergic rhinitis of rats

作　　者：邢志敏[1] 刘晓玲[1] 余力生[1] 王旻[1] 

机构地区：[1]北京大学人民医院耳鼻咽喉科,北京100044

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第11期506-509,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：科技部十一五科技支撑计划课题“变应性鼻炎规范化诊断和防治体系的研究”(No:2007BA118B115)

摘　　要：目的:探讨大气可吸入颗粒物(PM10)在激发大鼠变应性鼻炎过程中所起的作用及其可能的机制。方法:使用卵清蛋白(OVA)给予大鼠腹腔注射予以基础致敏,致敏结束后3个实验组分别用单独OVA、PM10+OVA联合及单独PM10鼻腔滴入予以激发,对照组用生理盐水代替OVA致敏,生理盐水激发,方法同实验组。观察并记录各组大鼠在激发过程中变应性鼻炎的症状,末次激发后24h处死大鼠,取鼻黏膜行免疫组织化学染色检测IL-4的表达情况。结果:OVA组和PM10+OVA组均诱发出明显的变应性鼻炎症状,而PM10组和对照组偶有搔鼻症状;症状积分PM10+OVA组与OVA组比较差异具有统计学意义(P<0.01),并分别与对照组比较差异均有统计学意义(均P<0.01);PM10组与对照组比较差异无统计学意义(P>0.05)。IL-4阳性表达的细胞计数3个实验组与对照组比较差异均有统计学意义(P<0.01),3个实验组之间比较存在显著性差异,PM10+OVA组明显高于OVA组(P<0.01)。结论:PM10在激发大鼠变应性鼻炎的过程中与变应原共同作用可以增强变应性炎症反应,加重大鼠变应性鼻炎的症状。Objective：In order to study the effect of inhalable particles on provocation of rat allergic rhinitis model and its possible mechanism.Method：After basicly sensitizing the experimental rats with OVA by intraperitoneal injection,three groups were provocated by solo OVA,PM10＋OVA and solo PM10 through nasal cavity respectively.The control group was sensitized by NS instead of OVA,and then provocated by NS as the method of experimental groups.The symptoms of rat during the provocation were observed and recorded.Then,the rats were sacrificed 24 hours after the last provocation,and the expression of IL-4 in nasal mucosa were detected by immunohistochemical method.Result：The groups of OVA and PM10＋OVA both had provocated the obvious symptoms of allergic rhinitis,while the groups of PM10 and NS had the symptoms of scratching nose occasionally.By statistical analysis of the amount of IL-4 positive cells,there were significant differences between each experimental group and NS group,and there was significant difference between three experimental groups.The amount of IL-4 positive cells in PM10＋OVA group was obviously higher than that in other groups.Conclusion：In the period of provocating the rat allergic rhinitis,the inhalable particles play a synergic role with allergen,and it can aggravate the symptoms of allergic rhinitis.

关 键 词：鼻炎 变应性 常年性 可吸入颗粒物 SD大鼠 白细胞介素-4 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]