科玛嘉念珠菌培养基在儿童牙菌斑中白色念珠菌分离培养的应用  

Use of CHROMagar Candida medium for isolation of Candida albicans from dental plaque in children

作　　者：邱荣敏[1] 赵玮[1] 卢佳璇[1] 林艳[1] 罗伟[1] 高硕[1] 

机构地区：[1]中山大学附属口腔医院儿童口腔科,广州510055

出　　处：《实用口腔医学杂志》2011年第3期382-384,共3页Journal of Practical Stomatology

基　　金：广东省自然科学基金项目(编号:8151008901000076);广东省科技计划社会发展项目(编号:00316971120302088)

摘　　要：目的：探讨科玛嘉念珠菌培养基（CAC）对儿童牙菌斑中白色念珠菌分离培养的有效性。方法：分别采用CAC和沙保培养基（SDA）分离培养儿童龋损牙体组织及无龋牙体龈上菌斑中的白色念珠菌，通过生化鉴定及PCR技术等方法对分离菌株进行鉴别，运用卡方检验比较两种培养基对白色念珠菌的检出情况。结果：CAC和SDA对牙菌斑中白色念珠菌的检出率分别为35．3％、31．7％（P〈0．05）。培养24—48h后，CAC平板通过颜色判读白色念珠菌的结果与生化鉴定和PCR基因分型的结果一致。结论：CAC是一种快速、有效、特异性较强的念珠菌培养基，适合用于分离培养儿童牙菌斑中的白色念珠菌。Objective： To explore the efficacy of CHROMagar Candida medium in isolating Candida albicans from dental plaque in children. Methods： CHROMagar Candida medium （CAC） and Sabourand Dextrose Agar （SDA） were used to isolate Candida albi- i cans from caries tissue and supraginval dental plaque of sound teeth in children, respectively. The colonies were confirmed by bio- chemical identification and genotyping with PCR. The difference of isolation frequency of Cam]ida albicans between CAC and SDA was compared by chi-square test. Results：The isolation frequency of Candida albicans by CAC and SDA was 35.3% and 31.7% respectively（ P 〈 0.05 ）. After 24 - 48 h culture, Candida albicans growing on CAC could be judged by the color of colonies, and the resuh was in accord with the outcome of biochemical identification and genotype. Conclusion： CAC is a kind of fast, effective, and specific medium for isolating Candida albicans from dental plaque in children.

关 键 词：白色念珠菌 牙菌斑 科玛嘉念珠菌培养基 沙保培养基 

分 类 号：R446.5[医药卫生—诊断学]