云南省不同宿主H9N2感染调查和NA分子进化分析  

Analysis of infection and NA molecular evolution of avian H9N2 in different hosts in Yunnan province

作　　者：张泉鹏[1,2] 李作生[1] 鲍晓伟[1,3] 李乙江[1,3] 张富强[1] 郑钧丰[1] 王意银[1] 张应国 张文东 宋建领[5] 邱薇[1] 李刚山[1] 张向荣[1] 范泉水[1] 

机构地区：[1]成都军区疾病预防控制中心,云南昆明650032 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]云南农业大学动物科学技术学院,云南昆明650201 [4]云南省动物疾病预防控制中心,云南昆明650051 [5]云南省热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《中国兽医学报》2011年第6期828-834,共7页Chinese Journal of Veterinary Science

基　　金：云南省"十一五"重大科技攻关资助项目(2006NG24)

摘　　要：2006年6月-2007年10月,从云南省16个地州随机采集的各种家禽、家猪的喉气管和口腔棉拭子样品7 901份,应用RT-PCR结合血凝、血凝抑制试验和抗原捕捉ELISA,检测H9N2亚型禽流感感染情况,选择具有代表性的阳性样品,克隆NA全基因并进行基因序列分和推导氨基酸的糖基化位点分析。结果表明,云南省2006-2007年H9N2亚型禽流感病毒在全省14个地州均有流行,感染宿主包括鸡、鸭、鹅和猪;分离的19个毒株中的13个鸡源和1个鹅源毒珠的NA全基因长1 407bp,编码469个氨基酸,同源性为96.1%～99.6%,是目前云南省流行的优势亚群;3个鸭源毒株、1个鹅源和1个猪源毒株NA基因长1 398bp,编码466个氨基酸,5个非鸡源毒株的NA基因的同源性为99%,构成云南目前流行的另一分支,推导氨基酸第61～63位3个氨基酸的缺失是与优势分支的特征性区别;2个分支间NA基因同源性为90%,与国内外其他毒株比较神经氨酸酶基因序列具有多态性。7 901 samples collected from poultry and swine in the Yunnan province between 2006 and 2007 were detected with RT-PCR,HA,HI,ELISA,and NA genes in representative positive samples were cloned and analyzed.The results suggest that H9N2 subtype influenza virus was epidemic in 14 administrative division of Yunnan province and swine and goose infected with H9N2 subtype influenza virus were confirmed in Yunnan province first time.The homology of NA genes of 19 strains from different districts in Yunnan province was between 89%-100% and homology of deduced amino acid was 96.1%-99.6%.The 14 strains（including 1 goose source strain） have became predominant sublineages of which are 95% homology of NA gene（1 407 bp nucleotides,encoding 469 amino acids）.Four strains（three duck,one gooes and one swine source strains）of NA gene homology 99% is another sublineages,the deletion of three-amino-acids at 61-63aa in NA consisted of 466 amino acids is a characteristic difference with predominant sublineages.NA genes of H9N2 subtype of avian influenza virus in Yunnan province exists a polymorphism with other strains of domestic and abroad.

关 键 词：H9N2亚型禽流感病毒 神经氨酸酶基因 糖基化位 基因多态性 

分 类 号：S852.65[农业科学—基础兽医学]