重庆地区家畜附红细胞体分子流行病学调查及危险性因素  

Molecular epidemiological and risk factors of livestock Eperythrozoonosis in Chongqing

作　　者：聂奎[1] 周作勇[2] 周荣琼[2] 罗洪林[1] 唐成[1] 胡世君[2] 汤明 胡友兰 於剑[4] 

机构地区：[1]西南大学动物科技学院,重庆北碚400715 [2]西南大学荣昌校区动物医学系,重庆荣昌402460 [3]重庆市农业局动物防疫检疫处,重庆401147 [4]重庆市中药研究院,重庆400065

出　　处：《中国兽医学报》2011年第6期848-851,共4页Chinese Journal of Veterinary Science

基　　金：重庆市自然科学基金重点资助项目(CSTC;2006BA1009)

摘　　要：本试验对重庆地区牛、猪和羊附红细胞体进行分子流行病学调查及危险性因素分析。在2006—2008年期间,采用鲜血压片法、姬姆萨染色法和基于16SrRNA-PCR方法,共检测了307头份牛血样,检测阳性率分别为46%、48%和11%;检测猪血样1 658头份,检测阳性率分别为63%、68%和76%;检测羊血样1 191份,检测阳性率分别为61%、70.4%和16.1%。危险性因素分析结果显示,山区牛附红细胞体感染阳性率极显著高于丘陵地区(OR=16.03,95%CI=5.47～17.11)(P<0.01),山区猪和山羊附红细胞体感染阳性率显著高于丘陵地区(猪:OR=1.38,95%CI=1.06～1.79;羊:OR=1.43,95%CI=0.54～3.82)(P<0.05)。农户散养牛和猪的附红细胞体感染阳性率极显著高于规模化养殖(牛:OR=3.2,95%CI=1.48～6.89;猪:OR=2.28,95%CI=1.79～2.89)(P<0.01)。农户散养山羊附红细胞体感染阳性率高于规模化养殖场(OR=1.19,95%CI=1.09～1.54)(P>0.05),但差异不显著。In this study,we performed molecular epidemiological surveys and risk factors analysis of Eperythrozoonosis for cattle,pig and goat in Chongqing.Samples were collected from 2006 to 2008 and Eperythrozoon infection were detected by three different methods,including blood smear,Giemsa stain assay and 16S rRNA-PCR.The prevalence of Eperythrozoon infection was 46%,48% and 11% in cattle,61%,70.4% and 16.1% in goat,and 63%,68% and 76% in pig by blood smear,Giemsa stain assay and 16S rRNA-PCR methods,respectively.The risk factors analysis showed that positive rate of Eperythrozoonosis of cattle in mountain area was extremely significantly higher than that in hill area（OR = 16.03,95% CI = 5.47-17.11,P0.01）.Similarly,the positive rates of Eperythrozoonosis of pig and goat in mountain area were also significantly higher than those in hill area（pig： OR=1.38,95%CI =1.06-1.79;goat： OR= 1.43,95% CI=0.54-3.82）.In addition,positive rates of pathogens of cattle and pig in scattered-feeding livestock were extremely significantly higher than those in large-scale breeding livestock（OR=1.19,95% CI = 1.09-1.54,P0.05）.The positive rates of pathogens of goat in scattered-feeding livestock was also higher than that in large-scale breeding livestock,although the difference was not significant（P0.05）.

关 键 词：牛 猪 羊 附红细胞体 流行病学 危险性因素 

分 类 号：S852.61[农业科学—基础兽医学]