奶牛乳房炎金黄色葡萄球菌基因多态性分型试验  

Research on genetic polymorphism of Staphylococcus aureus isolated from bovine mastitis

作　　者：邓海平[1] 倪春霞[1] 蒲万霞[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医学报》2011年第6期891-894,898,共5页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2006BAD04A05)

摘　　要：利用随机引物AP-7,建立引物随机多态性扩增(RAPD)体系对71株引起内蒙古和贵州地区奶牛乳房炎的金黄色葡萄球菌分离株进行基因分型研究。结果表明,71株金黄色葡萄球菌均得到清晰的RAPD指纹图谱,扩增产物为2～9条带,产物大小为240～4 500bp。菌株共分为6个基因型,其中Ⅰ型17株(占23.9%)、Ⅱ型3株(占4.2%)、Ⅲ型33株(占46.5%)、Ⅳ型15株(占21.1%)、Ⅴ型2株(占2.8%)、Ⅵ型2株(占2.8%)。Ⅰ型为内蒙古地区的流行优势菌群,Ⅲ型为贵州地区的流行优势菌群。两地区各基因型菌株比例有明显差异,这可能与奶牛养殖业水平和环境差异有关。Randomly amplified polymorphic DNA（RAPD） with a oligonucleotide primers AP-7 was used for research on genetic polymorphism of 71 Staphylococcus aureus（S.aureus） strains isolated from dairy cattle in Inner Mongolia and Guizhou area.The result shows that all of the 71 S.aureus strains produced fingerprint distinctly and amplified out 2-9 electrophoresis strips which reveal DNA markers with sizes ranging from 240-4 500 bp.The S.aureus strains were separated into 6 genotype by fingerprint and 17 strains were typeⅠ（23.9%）,3 strains were type Ⅱ（4.2%）,33 strains were type Ⅲ（46.5 %）,15 strains were type Ⅳ（21.1 %）,2 strains were typeⅤ（2.8 %） and 2 strains were typeⅥ（2.8 %）.The preponderant S.aureus strains of inner mongolia was typeⅠand typeⅢ was preponderant genotype of Guizhou area.Difference of dairy farming level,geographical environment and using of antibiotic may be the reason of distinct discrepancy on proportion of different genotype strains in the two areas.

关 键 词：奶牛乳房炎 金黄色葡萄球菌 RAPD 基因型 

分 类 号：S855.1[农业科学—临床兽医学]